Anticonvulsant activity of Achillea nobilis and Momordica charantia in rats

J Pharm Pharmacogn Res 4(3): 107-114, 2016.

Original Article | Artículo Original

The potential anticonvulsant activity of the ethanolic extracts of Achillea nobilis and Momordica charantia in rats

[Actividad anticonvulsivante de extractos etanólicos de Achillea nobilis y Momordica charantia en ratas]

Gamal A. Soliman1,2*, Hasan Yusufoglu3, Irem Tatli-Çankaya4, Rehab F. Abdel-Rahman5, Serap Aarabaci Anul4, Galip Akaydn6

1Pharmacology Department, Pharmacy College, Prince Sattam Bin Abdulaziz University, Al-Kharj, P.O. Box 173. Kingdom of Saudi Arabia.
2Pharmacology Department, Veterinary Medicine College, Cairo University, Egypt.
3Pharmacognosy Department, Pharmacy College, Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia.
4Department of Pharmaceutical Botany, College of Pharmacy, Hacettepe University, Sihhiye, Ankara, Turkey.
5Department of Pharmacology, National Research Centre, Cairo, Egypt.
6Deapartment of Biology Education, College of Education, Hacettepe University, Beytepe, Ankara, Turkey.
Abstract

Context: Currently available antiepileptic drugs have debilitating adverse effects. Natural products and plants already used in traditional medicine can be a good place to start in the search for safer and more effective options.

Aims: To investigate the anticonvulsant potential of Achillea nobilis and Momordica charantia extracts in maximal electroshock (MES), as well as pentylenetetrazole (PTZ)- and strychnine nitrate (STN)- induced seizure models in rats.

Methods: For each model, eight groups of 21-day-old male Albino rats were used. The 1st group was kept as control, 2nd as standard (diazepam, 7.5 mg/kg); 3rd – 5th treated with A. nobilis (100, 200 and 300 mg/kg); and 6th – 8th administered M. charantia (100, 200 and 300 mg/kg). After 30 min, rats were exposed to a shock of 150 mA by a convulsiometer, via ear electrodes for 2 s (in MES test) or sc injection of PTZ (85 mg/kg) or STN (2.5 mg/kg).

Results: A. nobilis and M. charantia extracts (200 and 300 mg/kg) demonstrated dose-dependent anticonvulsant effect against MES-induced seizures. In the PTZ induced convulsion, A. nobilis and M. charantia (200 and 300 mg/kg) significantly slowed the commencement of convulsions and minimized the duration of seizures. A. nobilis (300 mg/kg) showed 60% protection in rats against STN induced seizures. In contrast, A. nobilis (100 and 200 mg/kg) and M. charantia (100, 200 and 300 mg/kg) showed no significant protection against STN-induced seizures in rats.

Conclusions: The results of the present study suggest that both extracts exhibited marked anticonvulsant activities.

Keywords: Achillea nobilis; anticonvulsant; Momordica charantia; pentylenetetrazole; seizures.

Resumen

Contexto: En la actualidad los fármacos antiepilépticos disponibles presentan numerosos efectos adversos. Los productos naturales y las plantas, utilizados en la medicina tradicional, pueden ser un buen punto de partida en la búsqueda de opciones más seguras y eficaces.

Objetivos: Investigar el potencial anticonvulsivo de extractos de Achillea nobilis y Momordica charantia en electroshock máximo (MES), así como en modelos de convulsiones inducidas por pentilentetrazol (PTZ) y nitrato de estricnina (STN) en ratas.

Métodos: Para cada modelo, se utilizaron ocho grupos de ratas albinas macho de 21 días de edad. El primer grupo se mantuvo como control, el segundo como referencia (diazepam, 7,5 mg/kg); del tercero-quinto tratados con A. nobilis (100, 200 y 300 mg/kg); y del sexto-octavo administrados con M. charantia (100, 200 y 300 mg/kg). Después de 30 min, las ratas se expusieron a un choque de 150 mA de un convulsiómetro por 2 s, a través de electrodos para los oídos (en la prueba MES) o inyección sc de PTZ (85 mg/kg) o STN (2,5 mg/kg).

Resultados: Los extractos de A. nobilis y M. charantia (200 y 300 mg/kg) demostraron efecto anticonvulsivo dependiente de la dosis contra las convulsiones inducidas por MES. En la convulsión inducida por PTZ, A. nobilis y M. charantia (200 y 300 mg/kg) disminuyeron significativamente el comienzo de las convulsiones y redujeron al mínimo la duración de éstas. A. nobilis (300 mg/kg) mostró una protección del 60% contra las convulsiones inducidas por STZ. En contraste, A. nobilis (100 y 200 mg/kg) y M. charantia (100, 200 y 300 mg/kg) no mostraron una protección significativa frente a ataques inducidos por STN.

Conclusiones: Los resultados del presente estudio sugieren que los dos extractos mostraron una marcada actividad anticonvulsivante.

Palabras Clave: Achillea nobilis; anticonvulsivante; convulsiones; Momordica charantia; pentilentetrazol.

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Citation Format: Gamal A. Soliman, Hasan Yusufoglu, Irem Tatli-Çankaya, Rehab F. Abdel-Rahman, Serap Aarabaci Anul, Galip Akaydn (2016) The potential anticonvulsant activity of the ethanolic extracts of Achillea nobilis and Momordica charantia in rats. J Pharm Pharmacogn Res 4(3): 107-114.
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© 2016 Journal of Pharmacy & Pharmacognosy Research (JPPRes)