Antimicrobials produced by C. amboinicus endophytic fungus

J Pharm Pharmacogn Res 8(4): 280-289, 2020.

Original Article

Antimicrobial activities of isoprene compounds produced by an endophytic fungus isolated from the leaves of Coleus amboinicus Lour.

[Actividades antimicrobianas de compuestos de isopreno producidos por un hongo endofítico aislado de las hojas de Coleus amboinicus Lour.]

Puji Astuti1*, Rollando Rollando2, Subagus Wahyuono1, Arief Nurrochmad3

1Pharmaceutical Biology Department, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
2Program of Pharmacy, Faculty of Science and Technology, Ma Chung University, Malang, Indonesia.
3Pharmacology and Clinical Pharmacy Department, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Abstract

Context: Medicinal plants have been reported to produce various bioactive molecules. In this study an endophytic fungus was isolated from the leaves of Coleus amboinicus Lour., a medicinal plant used in Jamu and the fungus was identified as Athelia rolfsii.

Aims: To isolate, identify and characterize bioactive compound present in ethyl acetate extract of fermentation broth and determine its antimicrobial activities.

Methods: The compound was isolated and purified by Preparative Thin Layer Chromatography. Antimicrobial activities were conducted by determining its IC50 and MBC (Minimum Bactericidal Concentration) values against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella typhi and Staphylococcus mutans. The structure of the bioactive compound was deduced by spectroscopic data.

Results: The bioactive compound displayed antimicrobial activities with IC50 of 0.86, 1.35, 1.33, 2.69, 1.9, 0.24 µg/mL and MBC values of 40, 40, 40, 40, 20, 20 µg/mL against S. aureus, E. coli, P. aeruginosa, B. subtilis, S. typhi, S. mutans, respectively. Based on IR, LC-MS, 13C-NMR, 1H-NMR data, the bioactive compound was suggested to consist of two compounds, methyl hemiterpenoate as a major compound and methyl, 2,3 diene-butanoate as the minor one.

Conclusions: The endophytic fungus isolated from the leaves of C. amboinicus produced antimicrobial agents which could be potential for further development.

Keywords: antimicrobial; Athelia rolfsii; Coleus amboinicus; endophyte; fungus.

Resumen

Contexto: Se ha informado que las plantas medicinales producen diversas moléculas bioactivas. En este estudio, se aisló un hongo endofítico de las hojas de Coleus amboinicus Lour., una planta medicinal utilizada en Jamu y el hongo se identificó como Athelia rolfsii.

Objetivos: Aislar, identificar y caracterizar el compuesto bioactivo presente en el extracto de acetato de etilo del caldo de fermentación y determinar sus actividades antimicrobianas.

Métodos: El compuesto se aisló y purificó por cromatografía preparativa de capa fina. Las actividades antimicrobianas se llevaron a cabo determinando sus valores de IC50 y MBC (concentración bactericida mínima) contra Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Salmonella typhi y Staphylococcus mutans. La estructura del compuesto bioactivo se dedujo por datos espectroscópicos.

Resultados: El compuesto bioactivo mostró actividades antimicrobianas con IC50 de 0.86, 1.35, 1.33, 2.69, 1.9, 0.24 µg/mL y valores de MBC de 40, 40, 40, 40, 20, 20 µg/mL contra S. aureus, E. coli, P. aeruginosa, B. subtilis, S. typhi, S. mutans, respectivamente. Basado en datos de IR, LC-MS, 13C-NMR, 1H-NMR, se sugirió que el compuesto bioactivo consistiera en dos compuestos, metil hemiterpenoate como compuesto principal y metil, 2,3 diene-butanoate como el menor.

Conclusiones: El hongo endofítico aislado de las hojas de C. amboinicus produjo agentes antimicrobianos que pudieran ser potenciales para un mayor desarrollo.

Palabras Clave: antimicrobiano; Athelia rolfsii; Coleus amboinicus; endófito; hongo.

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Citation Format: Astuti P, Rollando R, Wahyuono S, Nurrochmad A (2020) Antimicrobial activities of isoprene compounds produced by an endophytic fungus isolated from the leaves of Coleus amboinicus Lour. J Pharm Pharmacogn Res 8(4): 280–289.

© 2020 Journal of Pharmacy & Pharmacognosy Research (JPPRes)