C 020: OZONE CONDITIONING EFFECT, NRF2 PATHWAY AND EPRE. AN IN VIVO STUDY DURING MAYOR AUTHOHEMOTHERAPY

J Pharm Pharmacogn Res 2(Suppl. 1): S49, 2014

Special supplement with the abstract book of LATINFARMA 2013

Conference

C 020: OZONE CONDITIONING EFFECT, NRF2 PATHWAY AND EPRE. AN IN VIVO STUDY DURING MAYOR AUTHOHEMOTHERAPY

Re L,1, Martínez-Sánchez G2, Bordicchia M3, Malcangi G1, Pocognoli A3, Morales-Segura M4, Rothchild J5 and Rojas A.6

1Clinical Pharmacology & Toxicology Dept, Medinat SAS, Via Fazioli 22, 60021 Camerano, Ancona, Italy. Tel. +39 071 731076 Fax. +39 071 731347, E-Mail: lambertore@lambertore.com
2Medical Center Beauty Benefit – San Biagio di Osimo, Via Mons. Oscar Romero, 31 60027 Osimo, Ancona, Italy. Tel. +39 3483833064, E-Mail: gregorcuba@yahoo.it
3Department of Internal Medicine, University of Ancona, Politecnica delle Marche, 60131 Ancona, Italy.
4Dept. Pharmacology, Faculty of Medicine, University of Chile, Santiago de Chile, Chile.
5Holistic Dentist Clinic, 175 Mercado Street Suite115, Durango, CO 81301, USA.
6Biomedical Research Laboratories, Medicine Faculty, Catholic University of Maule, Talca, Chile.
Abstract

Despite the long experience in the field of medical ozone therapy, and its positive clinical effects when used at appropriate concentrations, the most intimate molecular mechanisms are still partially unknown. The aim of our ongoing study, of which the present represents only the first step, was to verify some hypotheses to study on humans the involvement of the EpRE (electrophile-responsive element) system. Six adult healthy subjects of both gender and Caucasian ethnicity were eligible to participate in the study. Mayor Auto-hemotherapy (MAH) was performed, using a dose of ozone of 45 μg/kg of BW with ozone concentrations of 35 μg/mL. Patients were treated with 3 MAHs, one every 2 days interval. Blood samples for biochemical analysis were obtained after a 12 h overnight fast, at: 1) Immediately before the first MAH, 2) From the bottle during the first MAH after bubbling blood with ozone, 3) From the patients 30 min after the first MAH, 4) From the patients the day after the application of the third MAH. Periphery lymphocytes were isolated to assay Nrf2 levels and erythrocytes lysate were obtained to measure oxidative stress mediators. Nrf2 binding activity was increased (p<0.01) in human lymphocytes directly exposed to O3 and also (p<0.005) in circulating lymphocytes after MAH. Nrf2 signal back to normal status during the time. The initial activation of Nrf2 induced by ozone increase the level of antioxidants enzymes. These data provide new evidences on the mechanism responsible for the induction of antioxidant enzymes expression by ozonated MAH, and have a practical and clinical importance as an approach to the treatment of patients targeted to the restoration of redox homeostasis.