C 045: SELECTIVE CYTOTOXIC ACTIVITY OF 1-O-UNDECYL GLYCEROL

J Pharm Pharmacogn Res 2(Suppl. 1): S123, 2014

Special supplement with the abstract book of LATINFARMA 2013

Conference

C 045: SELECTIVE CYTOTOXIC ACTIVITY OF 1-O-UNDECYL GLYCEROL

Hernández-Colina M1, Martín A2, Calero A, Del Toro G.1

1Pharmacology &Toxicology Dept., Pharmacy and Food Institute. Havana, Cuba. E-mail: marianhc@ifal.uh.cu
2Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Abstract

1-O-alkylglycerols exhibit several pharmacological properties, which include sperm motility improvement, antidrepanocitary, antiangiogenic and antitumor activity. The cytotoxic activity of this family of compounds has been reported previously by our workgroup, but it didn´t include the 11 carbon chain molecule, 1-O-undecylglycerol (C11). The aim of this work was to demonstrate the cytotoxic activity of several concentrations of C11 in tumor cell cultures of different histological origins (A549, A375, MCF-7, MDA MB231), and compare with normal cell cultures (Vero cells, human primary fibroblast, 3T3A31, 184B5). Cytotoxicity was measured by MTT assay, and GI50 was determined using curve interpolation in GraphPad Prism v5.0. Taking into account the lowest GI50, obtained in breast cancer cells (MCF-7, MDAMB231), impedance measured by Real Time Cell Analyzer X-Celligence® performance showed differential behavior and cell index for cancer and normal breast cells treated with C11. The levels of caspase-3 and Akt after 3 and 24h of treatment with C11 changed in MCF-7 cells with respect to normal cells. We concluded that C11 has selective and concentration dependent cytotoxic action on cancer cell culture, which is more prominent in breast cancer cells, and mediated by apoptotic activity and Akt modulation in MCF-7 cells. 1-Oundecylglycerol could be a good candidate for further studies in selective anticancer therapy.