J Pharm Pharmacogn Res 2(Suppl. 1): S18, 2014
Special supplement with the abstract book of LATINFARMA 2013
CO 034: DISSECTION OF THE VENTRAL NERVE CORD-DEEP ABDONIMAL FLEXOR MUSCLE SYSTEM OF THE FRESHWATER LOBSTER OF THE Cherax GENUS: AN IN SITU MODEL FOR TEACHING PHARMACOLOGY
Islas V, Martínez C, Alavez JS, Hernández A, Lazo RE.
Introduction: The publication of norms regarding the use of laboratory animals has limited the implementation of practices towards the teaching of pharmacology, arising the need to develop alternative experimental models. In the present work, an in situ model of the ventral nerve cord-deep abdominal flexor muscle system as a pharmacology-teaching tool was developed.
Material and methods: Five lobsters of the Cherax genus were dissected separately as follows: the lobsters were anesthetized by submerging them for 10 minutes in an ice-bath. Afterwards, the head, the cephalothorax, and other crustacean extremities were removed, placing what remained on top of a dissection table. The ventral nervous-abdominal flexor muscle system was isolated after removal of the shell and abdominal cuticle. The caudal end of the muscle was attached to a myograph and the nerves were electrically stimulated using a varying voltage of 0.1 mV – 110 V; any muscular contractions were recorded. The electrical stimulation was repeated two more times, once with the addition of a solution of 2% lidocaine, and the other with the addition of 2% pilocarpine.
Results: The central nervous cord and the deep flexor muscle were easily isolated. All nerve-muscle dissections presented flexor-muscle contractions after stimulation of the ventral nerve with voltages ≥ 40 V. The contractions were inhibited after adding licodaine, and they were favored in the presence of pilocarpine.
Conclusions: The simple nervous and muscular structure of the Cherax genus lobster facilitated the dissection of the ventral nerve-deep abdominal flexor muscle system. By electrically stimulating the system, positive muscular contractions were obtained starting with 40 V. The contractions were also studied under the presence of lidocaine and pilocarpine. The above work gives a suitable alternative model for the teaching of pharmacology.