J Pharm Pharmacogn Res 2(Suppl. 1): S26, 2014
Special supplement with the abstract book of LATINFARMA 2013
CO 047: ANTINOCICEPTIVE ACTIVITY OF MANGIFERIN IN FORMALIN PAIN
Espinoza de los Monteros-Zuñiga Aa, Cervantes-Durán Cb, Lozada-García MCa, Izquierdo-Sánchez Ta, Godínez-Chaparro Ba,c.
Introduction: Mangiferin is a glucoxylxanthone present in the aqueous extract of Mangifera indica L., the extract has shown multiple pharmacological effects as antioxidant, anti-inflammatory and antinociceptive effects. The present study was undertaken to determine the possible antinociceptive activity of mangiferin in rats, as well as its possible mechanisms of action.
Material and methods: Nociceptive behavior was quantiﬁed as the numbers of ﬂinches of the injected paw during 1-min per 5 min, up to 60 min after 1% formalin injection. Magniferin was given orally (1-30 mg/kg), locally (1-30 µg/paw in 50 µL) or intrathecally (1-30 µg/rat in 10 µL) 30 min and 10 min before, respectively, 1% formalin injection in the dorsum paw. Diclofenac was used as a positive control. Animals were administered with vehicle or methiothepin (30 µg/paw), naloxone (5-50 µg/paw), naltrindole (0.1-1 µg/paw), 5-GNTI (0.1-1 µg/paw), L-NAME (10-100 µg/paw), ODQ (5-50 µg/paw) or glibenclamide (10-100 µg/paw) were administrated 20 min before mangiferin (30 µg/paw) which was given 10 min before of 1% formalin injection.
Results: The ipsilateral local peripheral (1-30 µg/paw), intrathecal (130 µg/rat) and oral (1-30 mg/kg) administration of mangiferin produced a dose-dependent reduction in formalin-induced nociception. The antinociceptive effect of this drug was similar to induced by diclofenac after oral and local peripheral administration. The local peripheral antinociceptive effect in the formalin test was blocked by naloxone (50 μg/paw), naltrindole (1 μg/paw), 5-GNTI (1 µg/paw), L-NAME (100 μg/paw), (50 μg/paw) and glibenclamide (50 μg/paw), but not by methiothepin (30 μg/paw).
Conclusions: These data suggest, that the antinociceptive effect induced by mangiferin are mediated by the modulation of peripheral opioidergic system involving the activation of δ, κ, and probably μ, receptors, but not serotonergic receptors. Also suggests that mangiferin activates the NO-cyclic GMP-ATP-sensitive K+ channels pathway related to modulate local peripheral nociceptive effect in rats.