J Pharm Pharmacogn Res 2(Suppl. 1): S43, 2014
Special supplement with the abstract book of LATINFARMA 2013
CO 071: EARLY PHASE OF SYSTEMIC REACTION TO ORAL ASPIRIN CHALLENGE IS RELATED TO AN INCREASE IN THE LEVELS OF IL-6 AND THE PERCENTAGE OF EFFECTORS MEMORY T-CELLS
Pérez-Novo CA1, Świerczyńska-Krępa M2,3, Niżankowska-Mogilnicka E2, Sanak M2, De Ruyck N2, Holtappels G1, Szczeklik A2, Bachert C1.
Introduction: Systemic reactions have been suggested to be linked to the hypersensitivity reactions occurring in aspirin exacerbated respiratory disease; however the mechanism has not been elucidated yet. In this study we aim to study the T- cell response and release of inflammatory mediators at systemic level following oral aspirin challenge and their possible link with clinical reactions in patients with aspirin exacerbated respiratory disease (AERD).
Methods: Patients with nasal polyposis and asthma with (n=20) and without AERD (n=18) were orally challenged with aspirin in a double placebo controlled study. Peripheral blood mononuclear cells were isolated and serum samples were collected before and after placebo and aspirin oral challenges. Cells were analyzed for CD4, CD8, CD25, CD127, CD45RA and CD45RO surface markers by Flow cytometry. Serum levels of inflammatory mediators: cytokines, chemokines and lipid mediators were assayed by using the Bioplex (Luminex) technology and ELISA. Urinary LTE4, 9α, 11β-PGF2 were analysed by ELISA.
Results: At baseline, AERD patients showed significant higher levels of sIL5Ra, uLTE4 and percentage of CD4+, CD4+CD25+CD127pos and CD4+CD45RA-CD45RO+ but decreased levels of TGF-β1 and number of CD4+CD25+CD127neg cells. Serum concentrations of IL-8 and sIL5R-α were significantly elevated in patients reacting to a dose of aspirin lower than 250 mg (half of the cumulative dose of 500 mg) when compared to non-AERD subjects and patients reacting to the highest doses of aspirin administrated (500 mg). Oral aspirin challenge resulted in the release of IL-6, urinary LTE4 and in the increased of the number of CD4+CD45RA-CD45RO+ memory T-cells only in AERD patients but failed to reduce the levels of sCD40L as occurred in nonAERD subjects. The release of IL-6 was elevated in patients with bronchial and nasal reactions when compared to subjects showing only bronchial reactions.
Conclusion: In conclusion, we demonstrated that early phase of systemic reaction after oral administration of aspirin is characterized by the release of IL-6 and the recruitment of effector memory T-cells. This points out to a novel mechanism that has been already discussed in allergy, asthma but not in AERD.
Citation Format: Pérez-Novo CA, Świerczyńska-Krępa M, Niżankowska-Mogilnicka E, Sanak M, De Ruyck N, Holtappels G, Szczeklik A, Bachert C (2014) EARLY PHASE OF SYSTEMIC REACTION TO ORAL ASPIRIN CHALLENGE IS RELATED TO AN INCREASE IN THE LEVELS OF IL-6 AND THE PERCENTAGE OF EFFECTORS MEMORY T-CELLS. [abstract]. In: Proceedings of the LATINFARMA 2013; 2013 October 21–25; La Habana, Cuba: SCF; J Pharm Pharmacogn Res 2(Suppl. 1): S43.
© 2014 Journal of Pharmacy & Pharmacognosy Research (JPPRes)