OC-01: BIOACTIVE EXTRACTS OF CHILEAN NATIVE PLANT FOR THE TREATMENT OF ULCERS

J Pharm Pharmacogn Res 3(suppl. 1): S11, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-01: BIOACTIVE EXTRACTS OF CHILEAN NATIVE PLANT FOR THE TREATMENT OF ULCERS

Rodríguez-Díaz M1, Rodríguez S2, Sabando C2, Gutiérrez C2, Torres F1, Delgado JM1.

1Escuela de Química y Farmacia, Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile. Email: maite.rodriguez@unab.cl, maiterd1974@gmail.com
2Centro de Investigación de Polímeros Avanzados (CIPA), Concepción, Chile.

 

Introduction: Gunnera tinctoria Mol., known as giant-rhubarb, Chilean rhubarb, nalca or pangue, is a continental specie distributed from Coquimbo to Magallanes, Chile. In our research, we develop biofilms starch/pectin functionalized with extracts from native Chilean plant. We carry out a phytochemical and pharmacological screening (in vitro antioxidant activity and in vivo anti-inflammatory and analgesic activities) from leaves extracts of Gunnera tinctoria Mol. Material and Methods: 100 g of the dried plant material was prepared by maceration for 48 h at room temperature with solvents of increasing polarity: hexane, dichloromethane, ethyl acetate, methanol, ethanol and water. Phenols quantification was carried out according to the Folin-Ciocalteu method. Topical anti-inflammatory activity was assessed in mice by TPA assay as inflammatory agent. To determinate the antinociceptive effect hot plate test was performed. Evaluation of antioxidant effect in vitro was performed using the FRAP and DPPH assays. Functionalized biofilms were standarized and tested pharmacologically. Results: Methanol extracts showed antioxidant activity (3.31 ± 0.083 mMFe2+/g), although the activity exhibited by this sample is less than that presented by quercetin (7.71 ± 0.198 mMFe2+/g sample) used as reference compound. Both the anti-inflammatory analgesic effect as statistically significant effects, the ethyl acetate extract show the topical antiinflammatory activity higher (54.7%) versus methanol extract (45.0%). Conclusions: The methanol extracts obtained from leaves of Gunnera tinctoria Mol. showed antioxidant, analgesic and anti-inflammatory activity, allowing support the properties attributed by folk medicine for this species. We develop biofilms functionalized with extracts of nalca.

Citation Format: Rodríguez-Díaz M, Rodríguez S, Sabando C, Gutiérrez C, Torres F, Delgado JM (2015) Bioactive extracts of Chilean native plant for the treatment of ulcers. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S11. Abstract nr OC-01.