OC-02: VASODILATOR EFFECT OF ALCOHOLIC EXTRACT OF THE LEAVES AND STEMS OF Oenothera rosea AIT.

J Pharm Pharmacogn Res 3(suppl. 1): S12, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-02: VASODILATOR EFFECT OF ALCOHOLIC EXTRACT OF THE LEAVES AND STEMS OF Oenothera rosea AIT. “YAWAR SUQU” IN ISOLATED AORTIC RINGS OF GUINEA PIGS, AYACUCHO-2013

Tinco J, Pariona F.

Facultad de Ciencias de la Salud, Universidad Nacional de San Cristóbal de Huamanga, Ayacucho, Perú. E-mail: aldotinco2@hotmail.com

Objectives: Evaluating the vasodilating effect of alcoholic extract of the leaves and stems of Oenothera rosea Ait. “yawar suqu” in isolated aortic rings of “guinea pigs”. Methods: Design: Experimental. Location: Environments of Pharmaco-logy and Pharmacognosy, School of Vocational Training of Pharmacy and Biochemistry of the Faculty of Health Sciences of the National University of San Cristobal de Huamanga, Ayacucho, Peru. Biological material: Leaves and stems of Oenothera rosea Ait. “yawar suqu” and aortic rings Cavys “guinea pigs”. Interventions: An extract of the leaves and stems of O. rosea was obtained. Macerated for 8 days in 70% ethanol, and subsequent concentration of the product. We worked with aortic rings of guinea pigs in camera isolated organs bathed with Krebs – Hensleit (KH) solution and vasomotor activity was recorded with an isometric tension transducer. Maximum basal contraction occurred with adrenaline (adrenergic α)  on which the vasodilator effect of three concentrations of the extract was determined: 5, 10 and 20%. Inhibiting vasoconstriction compared after incubation for 30 min extract 5, 10 and 20% and propranolol , β-blocker as the standard of comparison was used. Main outcome measures: Tension (g) and height (mm) of vasodilation and vaso-constriction. Results: In the phytochemical march moderate presence of flavonoids, phenols, tannins and mild form amino acids, quinones, saponins, triterpenoids or steroids were identified. A reduced pressure (maximum contraction) of 1.24 was observed to 1.38 g; 1.14; 1.28, concentrations of 5, 10 and 20%, respectively. In addition, reduced height (mm) of 6.85 to 6.15; 5.72, 6.37 mm for 5, 10 and 20% concentrations. Conclusions: The alcoholic extract of the leaves and stems of O. rosea produces vasodilation, whose greatest effect is observed at a concentration of 10%, and compared with standard propranolol similarity observed in the magnitude of vasodilation produced.

Citation Format: Tinco J, Pariona F (2015) Vasodilator effect of alcoholic extract of the leaves and stems of Oenothera rosea Ait. "yawar suqu" in isolated aortic rings of guinea pigs, Ayacucho-2013. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S12. Abstract nr OC-02.