OC-04: CHARACTERIZATION OF THE ANTINOCICEPTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT FROM Clusia minor L. LEAVES IN DIFFERENT EXPERIMENTAL MODELS IN MICE

J Pharm Pharmacogn Res 3(suppl. 1): S14, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-04: CHARACTERIZATION OF THE ANTINOCICEPTIVE ACTIVITIES OF THE ETHANOLIC EXTRACT FROM Clusia minor L. LEAVES IN DIFFERENT EXPERIMENTAL MODELS IN MICE

Reynaldo G1, de Souza MM2, Malheiros A2, Dallas MT2, Aver K2, Piovesan LG2, Rodeiro I1, Bello A1, Mangas R1, Menéndez R2.

1Instituto de Farmacia y Alimentos (IFAL). Universidad de La Habana. Cuba.
2Centro de Bioproductos Marinos (CEBIMAR). Agencia de Medio Ambiente, La Habana. Cuba. E-mail: rmenendez@cebimar.cu, roberto.menendez@infomed.sld.cu
3Universidade do Vale do Itajaí, Santa Catarina, Brazil.

 

Introduction: Despite advances in pain treatment, many patients do not achieve optimal pain relief from the existing medications and suffer side effects. Naturally originated agents possibly will substitute chemical therapeutics agents. We previously described that the ethanolic extract from Clusia minor L. leaves exhibits antinociceptive effects in the acetic acid-induced writhing and hot plate test. These results merit achieving new research to increase evidences of its anti-nociceptive action. With this aim, we studied the effects of C. minor extract on the formalin and capsaicin paw licking acute test and on carrageenan (Cg, 300 µg/paw), tumor necrosis factor (TNF, 50 pg/paw) α and prostaglandin E2 (PGE2, 100 ng/paw) induced mechanical hypernociception. Material and Methods: Plant extract (100, 150 and 300 mg/kg, p.o) or vehicle and control drugs (n = 6-7 each) was administered 30 min before the algesic stimulus. Results: The extract did not alter the motor activity of mice in the rota-rod or the open field models. The second phase of the nociceptive response induced by formalin was inhibited by the extract (100, 150 and 300 mg/kg) whereas, as occurred in hot plate, the first phase of formalin was inhibited only by the highest dose assayed.  Three hours after the injection of the inducer agents, the extract inhibited (150 and 300 mg/kg) the mechanical hypernociceptive response induced by Gg and those induced by TNFα and PGE2 (300 mg/kg). The phytochemical study revealed the presence Vit. E, phytosterols and the triperpenoid α-amyrin as the major components of the extract and the presence of the triterpenoid β-amirin, friedelin and lupeol as minor components. Thus, it is reasonable to conclude that the antinociceptive properties of C. minor extract occur via interaction of a variety mechanism that probably occurs via central and peripheral pathways modulating the inflammatory and neuropathic pain.

Citation Format: Reynaldo G, de Souza MM, Malheiros A, Dallas MT, Aver K, Piovesan LG, Rodeiro I, Bello A, Mangas R, Menéndez R (2015) Characterization of the antinociceptive activities of the ethanolic extract from Clusia minor L. leaves in different experimental models in mice. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S14. Abstract nr OC-04.