OC-06: Cymbopogon citratus FRACTIONS, PROTECTIVE ACTIVITY AGAINST UVC LIGHT IN Caulobacter crecentus

J Pharm Pharmacogn Res 3(suppl. 1): S16, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

OC-06: Cymbopogon citratus FRACTIONS, PROTECTIVE ACTIVITY AGAINST UVC LIGHT IN Caulobacter crecentus

Fuentes-León F, García F, Aguilera K, González-Pumariega M, Sánchez-Lamar A.

Dpto de Biología Vegetal, Facultad de Biología, UH. Calle 25 #445 e/I y J. Vedado, Plaza de la Revolución, La Habana, Cuba. Fax: (0537)832-132. Teléfono: 8328542. E-mail: fabiana@fbio.uh.cu

Introduction: Ozone layers hole entail the increase in ultraviolet radiation of sunlight on the earth surface, raising the risk of skin diseases, even cancer, in exposed individuals. The use of vegetal compounds is a new strategy to protect against UV damage by overexposure. Substancies obteined from natural sources are less hazardous than synthetic products. They also have a huge molecular diversity and biological function. Cymbopogon citratus (DC) Stapf, also known as “caña santa”, is consumed as a popular decoction and has several medicinal properties such as: antiinflammatory, analgesic, antispasmodic, hypotensor, anxiolytic, expectorant and antiasmatic. Other studies have also shown anticancer, antitumor and antimutagenic properties. A lot of these studies were assayed in total extract, less in fractioned extract. In this work, we tested the toxicity and the ability to protect against UVC light of three fractions obtained from Cymbopogon citratus (aqueous, butanolic and essential oils fractions). Material and Methods:  SOS Chromotest, Rifr test and Survival test were used in the experimental model of Caulobacter crecentus (NA 1000 p3213 lac z). Increasing concentrations of these fractions (0.1, 0.5, 1, 2 and 4 mg/mL) were applied continuously: before, during and after UVC exposure (45 J/m2). Results: Butanolic fraction posses cytotoxic and genotoxic properties. Aqueous fraction is not toxic but don´t protect DNA from UVC damage induced. Essential oils were cytotoxic at the highest concentration tested (4 mg/mL) but don´t possess genotoxic properties. This fraction decreases the frequency of mutants induced by UVC light in Caulobacter. Finally, essential oils showed antimutagenic activity and protect cells from radiation UVC mutagenicity.

Citation Format: Fuentes-León F, García F, Aguilera K, González-Pumariega M, Sánchez-Lamar A (2015) Cymbopogon citratus fractions, protective activity against UVC light in Caulobacter crecentus. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S16. Abstract nr OC-06.