OC-19: EVALUATION OF THE CONTENT OF VITAMIN C IN Malpighia glaba L.

J Pharm Pharmacogn Res 3(suppl. 1): S37, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-19: EVALUATION OF THE CONTENT OF VITAMIN C IN Malpighia glaba L. (ACEROLA)

García CM, Martínez V, García L, Nogueira A, González ML, Rivera MM, Sánchez P, Rodríguez C, Díaz M.

Center for Pharmaceutical Research and Drug Development (CIDEM). Ave. 26 # 1605 e/ Boyeros y Puentes Grandes. Havana, Cuba. E-mail: caridadgp@infomed.sld.cu, caridad.garcia@cidem.sld.cu

 

Introduction: To acerola (Malpighia glaba L.) have been attributed different therapeutic effects among which may be mentioned: as immunostimulant, detoxifying, mineralizing, among others, due to the high content of vitamin C in fruit. Material and Methods: The plant material was supplied by the Experimental Station of Medicinal Plants Dr. Juan Tomas Roig, while the aqueous extracts were prepared in the department of Pharmaceutical Technology, both belonging to CIDEM. In order to evaluate the content of vitamin C an analytical method was developed by High Performance Liquid Chromatography (HPLC) using a column LiChrosorb RP 18 (5 um) (250-4), a volume of injection of 20 uL, and a wavelength of 254 nm. The mobile phase consisted of a mixture of sodium heptanesulfonate: acetic acid: triethylamine. Validation parameters of specificity, accuracy, linearity, accuracy, detection and quantitation limits were determined. Results: The results obtained for every parameter evaluated, showed the reliability of the method developed and their applicability in controlling the quality and stability of the extracts.  The specificity study demonstrated that the method could quantify vitamin C in the presence of the degradation products and other components of the extract; while in the precision study it became clear that there were no significant differences between the means and variances obtained by analysts on different days. Conclusions: The analytical method developed for the quantification of vitamin C in Malpighia glaba L. cultivar `miguel´, was specific, precise, linear, accurate in the range of concentrations studied, demonstrating its applicability in the quality control and stability study.

 

Citation Format: García CM, Martínez V, García L, Nogueira A, González ML, Rivera MM, Sánchez P, Rodríguez C, Díaz M (2015) Evaluation of the content of vitamin C in Malpighia glaba L. (acerola). [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S37. Abstract nr OC-19.