OC-21: EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF Maytenus elaeodendroides GRISEB

J Pharm Pharmacogn Res 3(suppl. 1): S39, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-21: EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF Maytenus elaeodendroides GRISEB AND METABOLIC FINGERPRINT USING FIA/ESI/MSN

Spengler I1, García T1, Heredia E2, Fernández A1, Vilegas W3, Suárez Y1, Pérez C1.

1Center for Natural Product Studies, Faculty of Chemistry, Havana University, Cuba. E-mail: iraida@fq.uh.cu
2LIORAD Laboratory, BioCubaFarma, Havana, Cuba.
3Experimental Campus of Sao Vicente, UNESP—Sao Paulo State University, Sao Vicente, Sao Paulo, Brazil.

 

Introduction: The species of the Celastraceae family have a long history in traditional medicine. In the past 30 years, a large number of secondary metabolites with a wide range of biological activity has been extracted from Maytenus genus, including flavonoids and pentacyclic triterpenes. Many plants of this sort are used in South America to prepare infusions or decoctions as anti-inflammatory remedies for oral and topical administration. Material and Methods: The n-hexane extract (rich in triterpenes) and ethyl acetate (rich in flavonoids and especially catechin derivatives) obtained from Maytenus ilicifolia leaves showed high antiinflammatory and antiulcer activity. Extracts of M. ilicifolia and M. senegalensis are used in several countries to formulate drugs for these purposes; in this study, we set aim to evaluate the anti-inflammatory activity ethyl acetate of the bark of endemic species Maytenus elaeodendroides Griseb and obtaining their metabolic fingerprinting using direct flow injection electrospray ionization tandem mass spectrometry (FID/ESI/MSn). The dried ground plant material was extracted by maceration using diethyl ether/petroleum ether 1:1 and subsequently with ethanol. The ethanolic crude was dissolved in a hydroalcoholic solution and extracted with ethyl acetate. The anti-inflammatory activity assays were performed in vivo using the model of acute inflammation, ear edema. The metabolic fingerprint of the crude of ethyl acetate was obtained using a Thermo Finnigan LCQ Deca ion trap mass spectrometer (San Jose, CA, USA) equipped with an ESI interface. Results and Conclusions: The extract has anti-inflammatory activity and the metabolic fingerprint shows that the extract is rich in catechins derivatives and proanthocyanidins, some of which have novel structures.

 

Citation Format: Spengler I, García T, Heredia E, Fernández A, Vilegas W, Suárez Y, Pérez C (2015) Evaluation of anti-inflammatory activity of Maytenus elaeodendroides Griseb and metabolic fingerprint using FIA/ESI/MSN. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S39. Abstract nr OC-21.