OC-22: PHYTOCHEMICAL CHARACTERIZATION AND PRELIMINAR ANTINOCICEPTIVE ACTIVITY OF ETHANOLIC EXTRACT LEAVES OF Clusia minor L.

J Pharm Pharmacogn Res 3(suppl. 1): S40, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-22: PHYTOCHEMICAL CHARACTERIZATION AND PRELIMINAR ANTINOCICEPTIVE ACTIVITY OF ETHANOLIC EXTRACT LEAVES OF Clusia minor L.

Mangas R1, Reynaldo G1, Menéndez R2, Montes de Oca R3, Rodeiro I2, Bello A, de Souza MM4, Malheiros A4.

1Institute of Pharmacy and Food (IFAL), University of Havana. Ave. 23, 21425, La Lisa, Havana, Cuba. E-mail: raisam@ifal.uh.cu
2Center of Marine Bioproducts (CEBIMAR).
3Antidoping Laboratory, 100 Street and Aldabó. Havana. Cuba.
4Universidade do Vale do Itajaí (UNIVALI). Rua Uruguai, 458, 88302-202, Itajaí, SC, Brasil.

 

Introduction: The phytochemical studies of the genus Clusia (Clusiaceae) has demonstrated that constitute an important and rich source of metabolites secondaries. Material and Methods: In the present work we proposed the characterization of the principles compounds presented in the ethanolic extract leaves of Clusia minor L. by Gas Chromatography – Mass Spectrometry. Results: This analysis led the identification of nine compounds, principally, triterpenes, steroids and vitamin E. The most abundant compounds were γ-sitosterol (9.61%), mixture α-amyrin-lupeol (7.48%), vitamin E (6.62%) and β-amyrin (4.88%). Antinociceptive activity was evaluated using acetic acid-induced abdominal writhing (0.6%) and hot-plate tests. The acute oral administration of the extract (100, 150 and 300 mg/kg) increased significantly the number of abdominal contortions in the model employed to the all doses with 51, 67 and 32% of inhibition, respectively. In the hot-plate test only the higher dose tested have significant effect. Conclusions: These results show that the extract has antinociceptive effect and that this effect could be produced by inhibition of nociception of inflammatory origin or related directly with the stimulation of the afferents fibers where is not directly related to an inflammatory process. This effect could be attributed to the presence of the identified compounds, because some of them have described anti-inflammatory and antinociceptive activities. However, other components in the extract can contribute to this pharmacological activity.

 

Citation Format: Mangas R, Reynaldo G, Menéndez R, Montes de Oca R, Rodeiro I, Bello A, de Souza MM, Malheiros A (2015) Phytochemical characterization and preliminar antinociceptive activity of ethanolic extract leaves of Clusia minor L. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S40. Abstract nr OC-22.