OC-25: ANTIVIRAL ACTIVITY AND QUALITATIVE CHEMICAL COMPOSITION OF THE CUBAN PLANT Ageratina havamensis

J Pharm Pharmacogn Res 3(suppl. 1): S44, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-25: ANTIVIRAL ACTIVITY AND QUALITATIVE CHEMICAL COMPOSITION OF THE CUBAN PLANT Ageratina havamensis (KUNTH) R. M. KING & H. ROBINSON

García TH1, Quintino da Rocha C4, Dias MJ4, Pino L1, del Barrio G2, Roque A2, Pérez CE1, Campaner dos Santos L3, Spengler I1, Vilegas W4.

1Centro de Estudios de Productos Naturales, Facultad de Química, Universidad de La Habana. E-mail: trinhay@gmail.com; anaclaudia@fq.uh.cu
2Departamento de Microbiología y Virología, Facultad de Biología, Universidad de La Habana.
3Departamento de Química Orgánica, Instituto de Química de Araraquara, Universidad Estadual de São Paulo, Araraquara, São Paulo, Brasil.
4Campus Experimental del Litoral Paulista, Universidad Estadual de São Paulo, São Vicente, São Paulo, Brasil.

Introduction: The flowers and leaves of Ageratina havanensis (Kunth) R. M. King & H. Robinson are traditionally used as a tea to cure several diseases. The production of active secondary metabolites can be affected by several environmental factors such as climate, altitude, phonological status and other conditions that may influence the growth of plants. In this sense, the development of a methodology to compare chemical composition of plant extracts is a needed. Methods:  The cytotoxic effects of extracts in reproductive seasons on Vero cells were determined by the MTT colorimetric assay, as previously described. The influence of A. havanensis extract on cell viability was indirectly measured as a function of the ability of mitocondrial enzymes (active in viable cells) to convert MTT in a purple solid formazan precipitate. The antiviral activity of the extracts at noncytotoxic concentrations was assessed by measuring its protective effects on infected Vero cells. SI values greater than 2 were considered indicative of specific antiviral activity. The qualitative chemical composition of the plant collected in both reproductive and non-reproductive season was determinated by flow injection analysis-electrospray ionization-ion trap tandem mass spectrometry (FIA-ESI-IT-MSn) and ultra high-performance liquid chromatography coupled to electrospray positive ionization-ion trap mass spectrometry (UPLC/ESI-MSn). Results: The ethyl acetate extract was the most active and the qualitative chemical composition of  the extracts of the plant collected in flowering and non-reproductive seasons was very similar. Because of this, it can be expected that the extracts of the plant collected in non-reproductive season have antiviral activity. Conclusions: The antiviral activity of Ageratina havanensis is related to the presence of the flavonoids isolated previously and their synergic effect on the antiviral activity.

Citation Format: García TH, Quintino da Rocha C, Dias MJ, Pino L, del Barrio G, Roque A, Pérez CE, Campaner dos Santos L, Spengler I, Vilegas W (2015) Antiviral activity and qualitative chemical composition of the Cuban plant Ageratina havamensis (Kunth) R. M. King & H. Robinson. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S44. Abstract nr OC-25.