OC-28: ANTI-PLASMODIAL ACTIVITY OF Parthenium hysterophorus L. LACTONES

J Pharm Pharmacogn Res 3(suppl. 1): S47, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-28: ANTI-PLASMODIAL ACTIVITY OF Parthenium hysterophorus L. LACTONES

Fernández-Calienes A1, Fraga J1, Mendiola J1, Scull R2, Morier L1, Linares R1, Mendoza D1, Cuéllar A2.

1Departamento de Parasitología, Instituto de Medicina Tropical “Pedro Kourí” (IPK), Autopista Novia del Mediodía Km 6 ½, La Habana, Cuba, Apartado postal: 601 Marianao 13. E-mail: ayme@ipk.sld.cu
2Instituto de Farmacia y Alimentos. Universidad de La Habana. Calle 222  N°2317 entre 23 y 31, La Coronela, La Lisa, La Habana.

 

Introduction: Previous studies compilate information about plant species with reports of antimalarial use in Cuba and demonstrate antiplasmodial activity of crude extracts from Parthenium hysterophorus L. This plant produces a wide variety of sesquiterpenoid lactones, between them, parthenin. This lactone showed activity against Plasmodium faciparum. The purpose of this study was to evaluate antiplasmodial activity of lactones rich-extracts from root and aerial part of P. hysterophorus. Methods: Four extracts were prepared, two lactones rich-extracts from root and aerial part and one fraction without lactones each. Extracts activity evaluated in vitro against P. berghei and citotoxicity against human fibroblast MRC-5 was determined. The most selective extract was evaluated in vivo in a murine model. Results: Both lactones rich-extracts showed 50% of P. berghei schizogony development inhibition at 100 mg/mL  (aerial part 23.2 µg/mL and root 55.4 µg/mL), whereas, fractions “without  lactones” were inactives to that concentration. Root lactones rich-extract showed the highest selectivity index (IS = 3.1) although the value could be considered as mild. Intraperitoneal treatment with root lactones rich-extract of P. hysterophorus was no toxic to animals. It caused parasitemia significative reduction in mice treated with 1000 mg/kg. Conclusions: These results reaffirm antiplasmodial action of P. hysterophorus root and show lactones implication on this activity.

 

Citation Format: Fernández-Calienes A, Fraga J, Mendiola J, Scull R, Morier L, Linares R, Mendoza D, Cuéllar A (2015) Anti-plasmodial activity of Parthenium hysterophorus L. lactones. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S47. Abstract nr OC-28.