J Pharm Pharmacogn Res 3(suppl. 1): S48, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
OC-29: ANTIMALARIAL USE VALIDATION OF PLANT SPECIES IN CUBA
Fernández-Calienes A1, Fraga J1, Mendiola J1, Scull R2, Cuéllar A2, Bécquer E3, Herrera PP4.
Introduction: Malaria is the main parasitic disease causing high mortality and morbidity each year. Parasite resistance to current drugs threats malaria control. Based on traditional use and ethnomedical data, quina alkaloids and artemisinin were discovered in the past. Important ethnobotanical studies reveal traditional malaria treatments performed in Cuba before the disease eradication. This work is an attempt to become available a list of plants used empirically to combat malaria in Cuba, to summarise published data regarding the demonstration of their antimalarial activity and to identify natives species with antiplasmodial activity. Methods: A non-experimental validation of ethnomedical practices was aimed to point out traditional and probably effective treatments; three levels of validation were established: 1) for Cuban use only; 2) for uses recorded in other countries and 3) for positive antimalarial test. Antiplasmodial activity of 31 ethanolic extract was tested in vitro against Plasmodium berghei. Results: The use of 63 plant species for malaria, intermittent fevers and as quinine substitute were identify. Most represented family was Asteraceae, the majority of species were natives but only four were endemics. Decoction of leaves was the most frequent mode of remedy preparation and higher number of species was used in Eastern Cuba. Similar uses in other countries and/or positive screening against malaria parasites were reported for 36 of the 63 species. The medicinal use of 24 species possesses level 3 of non-experimental validation. Twenty-two extracts from 19 species (13 with level 3, 1 with level 2 and 5 with level 1) exhibited schizonts formation inhibition values upper than 50% at 100 µg/mL. Native species Baccharis halimifolia, Colubrina arborescens, Koanophyllon villosum, Oxandra lanceolata and Scutellaria havanensis demonstrated antiplasmodial activity for first time worldwide. Conclusions: Non-experimental validation and preliminary antiplasmodial test results substantiated the antimalarial use in Cuba for 30 plant species.
Citation Format: Fernández-Calienes A, Fraga J, Mendiola J, Scull R, Cuéllar A, Bécquer E, Herrera PP (2015) Antimalarial use validation of plant species in Cuba. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S48. Abstract nr OC-29.