J Pharm Pharmacogn Res 3(suppl. 1): S50, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
OC-31: INTERFACIAL AND ANTIMICROBIAL PROPERTIES OF THE EXOGENOUS PORCINE SURFACTANT MIXED WITH DRUGS USED IN RESPIRATORY CLINICS
Blanco O1, Pérez R1, Espinosa I1, Lugones Y1, Morilla A2, Faure R1.
Introduction: The excellent spreading properties of pulmonary surfactant suggest that the exogenous surfactant could be used as a drug delivery system to the alveolar compartment of the lung in the treatment of several lung diseases. However, it is vital that drugs delivered via lungs did not interfere with the pulmonary surfactant lining layer surface activity. The aim of this study was to evaluate the in vitro effect of N-acetylcysteine, hydrocortisone and antibiotics on the surfactant biophysical properties of SurfacenÒ (clinical pulmonary surfactant). Methods: The interfacial property of porcine lipid extract, Surfacen®, was evaluated in vitro by Langmuir balance. Measurements were obtained before and after the addition of a low and high concentration of drugs. In parallel, it was researched if Surfacen® affected the antibacterial activity of some antibiotic (amikacin, ampicillin, cefotaxime), by the time–kill studies of Escherichia coli and Staphylococcus aureus. Results and Conclusions: Drugs had no a significant effect on spreading kinetics of Surfacen®. Surfacen® interfered with amikacin activity in both E. coli and S. aureus. In contrast to the amikacin, the antibiotic activity of cefotaxime and ampicillin was not affect in vitro by the presence of SurfacenÒ. Future directions involve researches of these combinations in animal respiratory models.
Citation Format: Blanco O, Pérez R, Espinosa I, Lugones Y, Morilla A, Faure R (2015) Interfacial and antimicrobial properties of the exogenous porcine surfactant mixed with drugs used in respiratory clinics. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S50. Abstract nr OC-31.