OC-36: TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF Eugenia clarensis (BRITTON & P.WILSON), AN ENDEMIC CUBAN PLANT

J Pharm Pharmacogn Res 3(suppl. 1): S54, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-36: TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF Eugenia clarensis (BRITTON & P.WILSON), AN ENDEMIC CUBAN PLANT

Siverio-Mota D, Coba-Sánchez Y, Nguyen N, Vicet-Muro L, Jorge-Rodríguez ME, Sueiro-Oyarzun ML, González-Mosquera D, Herrera-Pis Y.

Department of Pharmacy. Faculty of Chemistry-Pharmacy, Universidad Central “Marta Abreu” de Las Villas, Santa Clara, 54830, Villa Clara, Cuba. E-mail: danys@uclv.edu.cu

 

Introduction: Antioxidant compounds can be useful to prevent several degenerative diseases or as preservative in food and cosmetic. Species of the Myrtaceae family are able to accumulate phenolic substances and those are closely related to the antioxidant activity due to their capacity to scavenge free radicals, chelate metals and protect against lipid peroxidation. Purpose: to investigate the total phenolic content and antioxidant capacity of ethanol, methanol and ethyl acetate extracts of the leaves of Eugenia clarensis (Britton & P.Wilson) an endemic plant to Villa Clara, Cuba. Methods: phenol and flavonoid content were determined by Folin Ciocalteu and AlCl3 reagent, respectively. The antioxidant activity of extracts were evaluated by employing various in vitro antioxidant assay: 1,1-diphenyl-2-picrylhydrazyl free radical scavenging (DPPH), total antioxidant activity determination by phosphomolybdenum method, ferric ion reducing antioxidant power (FRAP) and lipid peroxidation of linoleic acid emulsion by ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. Results: Ethanol extract showed the higher content of phenol and flavonoids with 246.44 mg equivalent to gallic acid/g of extract and 156.60 mg equivalent to rutin/g of extract, respectively. In addition, the ethanol extract exhibited the better result in DPPH method antioxidant (IC50=41.53 µg/mL) with strong antioxidant activities related to the standard BHT. Total antioxidant activity determination by ferric thiocyanate and FRAP assays showed the better result to ethanol extract too. The inhibitory action by FTC and TBA method determined the antioxidant capacity of ethanol extract. Conclusions: this study showed, for first time, the powerful antioxidant activity of Eugenia clarensis and demonstrated the potential benefits of specie on different diseases relative with oxidative stress.

 

Citation Format: Siverio-Mota D, Coba-Sánchez Y, Nguyen N, Vicet-Muro L, Jorge-Rodríguez ME, Sueiro-Oyarzun ML, González-Mosquera D, Herrera-Pis Y (2015) Total phenolic content and antioxidant activity of Eugenia clarensis (Britton & P.Wilson), an endemic Cuban plant. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S54. Abstract nr OC-36.