OC-40: In vivo ANTITUMOR ACTIVITY OF MANGIFERIN IN MURINE COLON CARCINOMA

J Pharm Pharmacogn Res 3(suppl. 1): S58, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-40: In vivo ANTITUMOR ACTIVITY OF MANGIFERIN IN MURINE COLON CARCINOMA

Rodríguez-González JC1, Hernández-Balmaseda I2, Quiñones-Maza OL1, Merino N1, Aparicio-López G1, Valdés-Martínez O1, Valentín-Quiñones N1, Gabilondo-Ramírez T1, Limonta-Ávalo R1, Rodeiro-Guerra I2, Beck Ilse M3, Wagemans G3, De Wever O3, Vanden Berghe W4, Delgado-Hernández R1.

1Center for Pharmaceutical Research and Drug Development (CIDEM). Ave.26 No. 1605, entre Puentes Grandes y Boyeros, Nuevo Vedado, CP 10600, Plaza, Havana, Cuba. E-mail: jcesar.rodriguez@cidem.sld.cu, rdelgado@infomed.sld.cu
2Laboratory of Pharmacology, Center of Marine Bioproducts. Loma y 39, Nuevo Vedado, C.P. 10600, Havana, Cuba. E-mail: ivones@cebimar.cu
3Laboratory of Experimental Cancer Research, Ghent University Hospital, Belgium.
4Laboratory of Protein Chemistry, Proteomics and Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp (UA), Antwerp, Belgium; Laboratory of Eukaryotic Gene Expression and Signal Transduction (LEGEST), Department of Physiology, Ghent University, Ghent, Belgium. E-mail: wim.vandenberghe@uantwerpen.be

 

Introduction: Colorectal cancer has high mortality due to recurrent and metastatic disease. Chemotherapy is the most commonly used therapy in these cases but is not effective enough and is limited by their adverse effects and chemoresistance. Natural products are important sources of antitumor compounds. Mangiferin, a natural antioxidant glucosylxanthone, has different pharmacological properties and its anticancer potential is just beginning to be elucidated. Objective: To evaluate the in vivo antitumor activity of mangiferin in CT-26.WT murine colon carcinoma syngeneic tumor models. Methods: Three syngeneic models ectopic/subcutaneous allograf, induced lung metastasis and tumor angiogenesis in matrigel plugs were performed. Target gene specific expression analysis by quantitative PCR assay was accomplished. Results: The antitumor and antimetastatic effect was positively correlated with increasing concentration of mangiferin, achieving the greatest effect at 100 mg/kg. In the tumor angiogenesis model, macroscopic observation showed a reduction of blood supply and tumor size, and a significant reduction in hemoglobin content into matrigel plugs was obtained by quantitative analysis at both concentration of mangiferin evaluated (100 and 200 ug/mL). The antiangiogenic effect had significantly impact on decreasing weight and tumor volume. Histological analysis revealed a decreased number of blood vessels. Conclusions: Mangiferin holds promise as an effective antitumor agent by triggering antimetastatic and antiangiogenic effects. Financial Support: VLIR UOS programme Nr. ZEIN2011PR383, Belgium and from Project of Cuban Ministry of Health nr. 1401049.

Citation Format: Rodríguez-González JC, Hernández-Balmaseda I, Quiñones-Maza OL, Merino N, Aparicio-López G, Valdés-Martínez O, Valentín-Quiñones N, Gabilondo-Ramírez T, Limonta-Ávalo R, Rodeiro-Guerra I, Beck Ilse M, Wagemans G, De Wever O, Vanden Berghe W, Delgado-Hernández R (2015) In vivo antitumor activity of mangiferin in murine colon carcinoma. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S58. Abstract nr OC-40.