OC-41: MANGIFERIN POTENTIATES PLATIN AND 5-FLUOROURACIL INDUCED CELL DEATH IN SIMPLE AND COMBINATION ANTINEOPLASTIC TREATMENTS

J Pharm Pharmacogn Res 3(suppl. 1): S59, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-41: MANGIFERIN POTENTIATES PLATIN AND 5-FLUOROURACIL INDUCED CELL DEATH IN SIMPLE AND COMBINATION ANTINEOPLASTIC TREATMENTS IN CT26.WT CELLS

Rodríguez-González JC1, Quiñones-Maza OL1, Hernández-Balmaseda I2, Valentín-Quiñones N1, Rodeiro-Guerra I2, Delgado-Hernández R1.

1Center for Pharmaceutical Research and Drug Development (CIDEM). Ave.26 No. 1605, entre Puentes Grandes y Boyeros, Nuevo Vedado, CP 10600, Plaza, Havana, Cuba. E-mail: jcesar.rodriguez@cidem.sld.cu, olgaq@infomed.sld.cu, rdelgado@infomed.sld.cu
2Laboratory of Pharmacology, Center of Marine Bioproducts. Loma y 39, Nuevo Vedado, C.P. 10600, Havana, Cuba.

 

Introduction: Mangiferin inhibits NF-kB, which is constitutively activated in cancer and is induced in response to chemotherapy and radiotherapy. Agent inhibitors of NFκB function might be considered as an adjuvant approach in combination with chemotherapy. Objective: To evaluate the chemosensitizer adjuvant effect in vitro of mangiferin in cotreatments with antineoplastic platinum and 5-fluorouracil. Methods: Mangiferin (1-200 μg/mL) was used in simple and combination (sequential and simultaneous) cotreatments of cisplatin, oxaliplatin and 5-fluorouracil at different times and at non cytotoxic concentrations in CT26.WT cells. The cell survival was evaluated by MTT assay. Results: In cytostatic simple cotreatments, the highest significant increases of cell death were obtained at lowest concentration of cytostatic (cisplatin 1 µM, 5-fluorouracil 0.1 µM). The best results were obtained in sequential cotreatment for cisplatin and in simultaneous cotreatment for 5-fluorouracil. Mangiferin not increase oxaliplatin-induced cell death. In cytostatic combination treatments with cisplatin/5-fluorouracil at low concentrations (1/0.1 µM) increased cell death was independent of the treatment sequence, instead at high concentrations (5/0.5 µM) there was a dependence on treatment schedule. The best results were obtained in the sequential combination at low concentrations. When mangiferin was combinated with oxaliplatin/5-fluorouracil the best result of all series of experiments was obtained by simultaneous cotreatment at low concentrations of cytostatic (1/0.1 µM) and high concentrations of mangiferin (100/200 µg/mL). Conclusions: Mangiferin in combination treatment both in simple and simultaneous cotreatments of cytostatic platinum with 5-fluorouracil potentiates cytostatic-induced cell death in our experimental conditions. Financial Support: Cuban Ministry of Health: grant from project nr.1008017.

Citation Format: Rodríguez-González JC, Quiñones-Maza OL, Hernández-Balmaseda I, Valentín-Quiñones N, Rodeiro-Guerra I, Delgado-Hernández R (2015) Mangiferin potentiates platin and 5-fluorouracil induced cell death in simple and combination antineoplastic treatments in CT26.WT cells. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S59. Abstract nr OC-41.