OC-42: Zuccagnia punctata EXTRACT AND 2’,4’-DIHYDROXYCHALCONE (DHC), A NATURAL FLAVONOID FROM THEM AS AN POTENTIAL ANTIMETASTATIC IN MURINE COLORECTAL MODEL

J Pharm Pharmacogn Res 3(suppl. 1): S60, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-42: Zuccagnia punctata EXTRACT AND 2’,4’-DIHYDROXYCHALCONE (DHC), A NATURAL FLAVONOID FROM THEM AS AN POTENTIAL ANTIMETASTATIC IN MURINE COLORECTAL MODEL

Zampini IC1,2,3, Francisco M4, Nuño G1, Carabajal A1,  Isla M1,2,3, Piloto-Ferrer J4.

1Laboratorio de Investigación de Productos Naturales (LIPRON), Instituto de Química del NOA (INQUINOA.CONICET).
2Facultad de Ciencias Naturales e IML. Universidad Nacional de Tucumán.
3Fundación Miguel Lillo. S.M. de Tucumán, Tucumán (4000) Argentina.
4Departamento de Toxicología especial, Centro de Investigación y Desarrollo de Medicamentos (CIDEM) La Habana (10400) Cuba. E-mail: marbelis.francisco@cidem.sld.cu

 

Introduction: Zuccagnia punctata is a shrub from Fabaceae family, which commonly grows distributed in arid and semiarid areas in central and western Argentina. This plant is used in ethnomedicine as antimicrobial, anti-inflammatory and antitumor drug and some biological properties were reported for their extracts, and have beneficial effect on some key mechanisms involved in the pathogenesis of cancer (antioxidant, antigenotoxic). The aim of the present study was to determine in vitro cytotoxic effects, using mammalian cell cultures, of ethanolic extract from Z. punctate (Zp) aerial part and four flavonoids isolated of them: 7-hydroxyflavanone (HF), 3,7-dihydroxyflavone (DHF), 2’,4’-dihydroxy-3’-methoxy-chalcone (DHMC) and 2’,4’-dihydroxychalcone (DHC), and to evaluate their antimetastatic effect using an in vivo model in mice. Material and Methods: The cytotoxicity of the natural products was tested using the MTT viability assay on two tumoral cell lines, MCF-7 and CT-26. The assays were performed at 24, 48 and 72 h. In addition, the anti-metastasic effect was evaluated in vivo using a CT-26 murine colorectal carcinoma model. Results: The results demonstrated that Z. punctata ethanolic extract and their flavonoids, induced cytotoxic effects on the tumoral cell lines evaluated. The activity on cell lines after 72 h of exposing was in the following order: DHC>DHCM>DHF>Zp and HF not showed cytotoxic effect. The active samples reduced cell viability in a concentration-dependent manner and the effect increased with the incubation time. In in vivo assay the oral administration of crude extract and the more in vitro cytotoxic compound, DHC, showed capacity to decrease the number of tumor nodules and lung volume after 14 day of administration of products once a day. Conclusions: These results may provide valuable information for further designing and developing of anticancer natural agents.

Citation Format: Zampini IC, Francisco M, Nuño G, Carabajal A, Isla M, Piloto-Ferrer J (2015) Zuccagnia punctata extract and 2’,4’-dihydroxychalcone (dhc), a natural flavonoid from them as an potential antimetastatic in murine colorectal model. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S60. Abstract nr OC-42.