OC-44: ASSESSMENT OF GENOTOXIC AND CYTOTOXIC EFFECTS OF ENDEMIC Phyllanthus PLANTS AQUEOUS EXTRACTS

J Pharm Pharmacogn Res 3(suppl. 1): S62, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-44: ASSESSMENT OF GENOTOXIC AND CYTOTOXIC EFFECTS OF ENDEMIC Phyllanthus PLANTS AQUEOUS EXTRACTS

Menéndez I, Sánchez-Lamar A.

Laboratorio de Genética Toxicológica, Departamento de Biología Vegetal, Facultad de Biología, Universidad de la Habana. Calle 25, # 455, e/ I y J, Vedado, Plaza de la Revolución, La Habana, Cuba. E-mail: ivette@fbio.uh.cu, alamar@fbio.uh.cu

 

Introduction: Plants of the genus Phyllanthus have been widely use by traditional medicine all over the world. In Cuba, there is a plentiful variety and endemism of them, and various species have proved antiviral, antioxidant and antimutagenic properties against chemical and physical mutagens. In order to extend the search for natural compounds presenting genoprotective properties to other species of this genus, previous studies about their possible genotoxic and cytotoxic properties must be done. Methods: In the present work, the aqueous extracts of three endemic Phyllanthus species were assessed: P. williamioides, P. chamaecristoides, and P. microdictyus, at concentrations ranging from 0.1 to 2 mg/mL and using Caulobacter crescentus cells as the in vitro experimental model. Genotoxicity was assessed through evaluating primary DNA damage by means of the SOS Chromotest assay. Cytotoxicity was measured through the survival assay, by the capacity of colony formation. Results: the genotoxic and cytotoxic effects were only statistically significant by way of a Dunnett Test (p<0.05), for the highest concentration tested and only in case of the P. williamioides plant extract. In all cases, the LD50 values were higher than the maximum concentration assayed, and P. chamaecristoides and P. microdictyus extracts showed a survival rate over 90%. Conclusions: These outcomes are in correspondence with preceding studies about the genotoxicity and cytotoxicity of several Phyllanthus species, through different experimental models, in vitro and in vivo, and they validate future researches about the possible antimutagenic properties of these plants aqueous extracts.

 

Citation Format: Menéndez I, Sánchez-Lamar A (2015) Assessment of genotoxic and cytotoxic effects of endemic Phyllanthus plants aqueous extracts. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S62. Abstract nr OC-44.