OC-45: ANTI-PROLIFERATIVE ACTIVITY OF FRACTIONS ISOLATED FROM Boldoa purpurascens CAV. IN HUMAN PROSTATE AND LUNG CANCER CELLS

J Pharm Pharmacogn Res 3(suppl. 1): S63, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-45: ANTI-PROLIFERATIVE ACTIVITY OF FRACTIONS ISOLATED FROM Boldoa purpurascens CAV. IN HUMAN PROSTATE AND LUNG CANCER CELLS

Hernández Y1, González DM1, Rodríguez T1, Tamargo B2, Sierra G3, Apers S4.

1Department of Pharmacy, Central University “Marta Abreu” of Las Villas, Cuba. E.mail: dulcem@uclv.edu.cu
2Institute of Pharmacy and Food, University of Havana, Cuba.
3BioCubaPharma, Havana, Cuba.
4Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Belgium.

 

Introduction: Boldoa purpuracens Cav. is a plant belonging to Nyctaginaceae family used in Cuba and other Latin America regions as diuretic. Phytochemical analysis has shown the presence of biologically active compounds such as flavonoids and triterpenic saponins in the plant. The aim of the present investigation was to evaluate the antiproliferative potential of a fraction of saponins isolated from stem of the species in H-460 and H-125 human lung cancer cells and PC-3 human prostate carcinoma grade IV. Methods: Dried and pulverized stem bark (1.2 kg) of Boldoa purpurascens were defatted and subsequently extracted with 80% ethanol. Ethanol extract was evaporated and extracted with water-saturated n-BuOH (crude of saponins). The crude was applied onto the Sephadex LH-20 column, using a mixture of chloroform/methanol/water (100: 10: 0 to 100: 100: 1) to get the fractions. Fraction 2 was used for the antiproliferative assay.  PC 3 (7, 5 x 104 cells/mL), H 460 (1 x 104 cells/mL), H 125 (1 x 104 cells/mL) and MCR5 (7.5 x 104 cells/mL) cells were seeded in 96-well plates. Concentrations of 100, 10, 0.1, 0.01 and 0.001 µg/mL of F2 were used and Quillaja saponaria, paclitaxel and cisplatin were used as standards. Absorbance was measured at 562 nm. Results: The high cell density assay showed IC50 values of 23.05, 133.99 and 13.06 µg/mL for PC-3, H-460 and H-125 tumor cancer cells, respectively. Conclution: the fraction of saponins isolated from stem bark of Boldoa purpurascens showed a potent antiproliferative effect.

 

Citation Format: Hernández Y, González DM, Rodríguez T, Tamargo B, Sierra G, Apers S (2015) Anti-proliferative activity of fractions isolated from Boldoa purpurascens Cav. in human prostate and lung cancer cells. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S63. Abstract nr OC-45.