OC-61: PRECLINICAL PHARMACOLOGICAL VALIDATION AND PHYTOCHEMICAL SCREENING OF Phania matricarioides

J Pharm Pharmacogn Res 3(suppl. 1): S22, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-61: PRECLINICAL PHARMACOLOGICAL VALIDATION AND PHYTOCHEMICAL SCREENING OF Phania matricarioides

Duménigo A, García AI, Martínez I, Victoria MC, Brito G, Blanco Y, Acosta L.

Central Laboratory of Pharmacology, Havana Medical Sciences University, Carvajal 156 entre Calle A y Diana. Cerro. Havana, Cuba. CP: 12000. E-mail: abeldg@infomed.sld.cu, abeldg@fallende.sld.cu

 

Introduction: Phania matricarioides is a plant that can be found throughout Cuba and is grown in gardens. The decoction or infusion from the fresh aerial parts is traditionally used to treat digestive and dermatologic problems. The aims of this work are: to evaluate the preclinical antinociceptive and anti-inflammatory action of the decoction of fresh aerial parts of P. matricarioides and the anti-inflammatory activity of five fractions obtained from this plant. Also was realized a phytochemical screening that allow supporting the pharmacological results. Material and Methods: The aerial parts of P. matricarioides were picked up to make decoction. Several pharmacological studies were conducted: the 0.75% acetic acid-induced contortions (0.1 mL/10 g, intraperitoneally administered); tail immersed in water at 55 ºC in mice, at doses of 1 and 5 g of vegetal material/kg of body weight; croton oil-induced ear oedema, orally (0.1 and 1 g/kg) and topically (10, 30 and 50 % concentrations) in mice and cotton-induced granuloma in rats (5 g/kg). The five fractions obtained were studied topically (0.25, 0.5, 1 y 2 mg/ear) in croton oil-induced ear oedema. Phytochemical screening of the plant was realized by Chabra´s method. Results: The decoction significantly inhibited the nociceptive response induced by acetic acid, depending on dose (5 g/kg), but did not affect either the tail withdrawal in immersion or the inflammatory response to cotton-induced granuloma; the inflammation treated orally and topically was inhibited in the ear oedema. Only the aqueous fraction showed topical anti-inflammatory activity. The chemical composition of the fresh aerial parts mainly comprised phenols, tannins, flavonoids and triterpenes or steroids. Conclusions: The results allowed performing the preclinical validation of the analgesic and anti-inflammatory action of the decoction of fresh aerial parts and of the aqueous fraction of this plant.

 

Citation Format: Duménigo A, García AI, Martínez I, Victoria MC, Brito G, Blanco Y, Acosta L (2015) Preclinical pharmacological validation and phytochemical screening of Phania matricarioides. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S22. Abstract nr OC-61.