J Pharm Pharmacogn Res 3(suppl. 1): S71, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
OC-68: BIOACTIVITY-GUIDED FRACTIONATION FOR ANTI-CANCER ACTIVITY OF Xanthium strumarium L. AND THEIR RESPONSIBLE CONSTITUENTS
Piloto-Ferrer J, Zampini IC, Cuello S, Francisco M, Romero A, González ML, Pasquale S, Fiore M, Sánchez-Lamar A, Isla M.
Introduction: Xanthium strumarium L. (Family: Asteraceae) a medicinal plant commonly found as a weed, is widely distributed in North America, Brazil, China, Malaysia and hotter parts of India. Xanthium strumarium L., have been used in traditional Cuban medicine as an effective diuretic drug. Internationally some others activities has been reported, e.g. antifungal, antiinflammatory, hypoglycemic, antioxidant, diuretic effects, by in particular, anti-tumour, anti-cancer activity, so much attention is focussed on the herb. The aim of this study was to fractionate an extract of X. strumarium (XSE) grown in Cuba to investigate the anticancer properties and the isolation of compounds potentially responsible for this activity. Material and Methods: The whole extract was subjected to a bioassay-guided multistep separation procedure, aiming at isolating the active molecule(s) responsible for its activity. Initially, an ethanol partitioning procedure yielded the XSE extract that was subsequently fractionated with chloroform resulting in a XSCF fraction. XSCF was subsequently fractionated by chromatography methods and was determined the cytotoxic and antimitotic effect against cancer cell lines. The active fractions subjected to semipreparative HPLC for isolation of bioactive compounds. The identification the compounds were by GC-MS. Results: Bioassay-guided fractionation of XSE led obtained six sub-fractions (SF1 to SF6). Sub-fractions SF1 and SF6 showed the strongest inhibitory effect. The HPLC-DAD fingerprint of SF6 showed a single peak that was identified by GC-MS as (-) spathulenol, while that SF1 showed three compounds identified as xanthatin, 8-epi- xanthatin and xanthinosin. Conclusions: The compounds (-) spathulenol, xanthatin, 8-epi- xanthatin and xanthinosin are responsible for the anticancer activity of the Cuban species of X. strumarium L.
Citation Format: Piloto-Ferrer J, Zampini IC, Cuello S, Francisco M, Romero A, González ML, Pasquale S, Fiore M, Sánchez-Lamar A, Isla M (2015) Bioactivity-guided fractionation for anti-cancer activity of Xanthium strumarium L. and their responsible constituents. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S71. Abstract nr OC-68.