J Pharm Pharmacogn Res 3(suppl. 1): S42, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
OC-82: Prosthechea karwinskii, EXTRACTION METHODS AND THEIR ANTIOXIDANT COMPOUNDS
Lagunez-Rivera L, Solano-Gómez R, Rojas-Olivos A.
Introduction: Orchids are appreciated for their ornamental value and traditional uses, and in many indigenous communities, they comprise part of a biocultural heritage. The orchid Prosthechea karwinskii is endemic to Southern Mexico where it grows as an epiphyte in oak or pine-oak forests. This plant has showy yellow flowers with a very pleasant aroma; it is particularly relevant because their medicinal and other traditional uses given by indigenous peoples, showing a promising future for ethnopharmacological research for this species. The goals of this study were 1) to identify antioxidant molecules present in phenolic extracts from P. karwinskii, and 2) evaluate the antioxidant activity and to known the phenols content of this species. Material and Methods: The phenolic fraction was obtained from dried seudobulbs and leaves of P. karwinskii using an extraction method with subcritical water. The antioxidant activity was evaluated by DPPH radical scavenging assay; total phenols ware quantified by a modified method of Folin‐Ciocalteu, and detection of phenolic compounds by HPLC-DAD. Results: The AAI and IC50 for P. karwinskii extracts were higher in comparison with values reported for other orchids. The total phenols content in the extract was 370 mg/g. There were identified seventeen phenolic compounds in the extract; the phenols with the highest concentration were hydroxytyrosol, tyrosol, peonidin, apigenin, and delfinidin; all they are compounds well known by their antioxidant properties. Conclusions: The compounds detected in this study reveal a promising potential of this species in the pharmaceutical, food, cosmetic, and perfume industries.
Citation Format: Lagunez-Rivera L, Solano-Gómez R, Rojas-Olivos A (2015) Prosthechea karwinskii, extraction methods and their antioxidant compounds. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S42. Abstract nr OC-82.