PACH-73: OPTIMIZATION OF EXTRACTION CONDITION FOR FLAVONOIDS IN Talipariti elatum S.W. USING RESPONSE SURFACE METHODOLOGY

J Pharm Pharmacogn Res 3(suppl. 1): S166, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PACH-73: OPTIMIZATION OF EXTRACTION CONDITION FOR FLAVONOIDS IN Talipariti elatum S.W. USING RESPONSE SURFACE METHODOLOGY

Barrios M, Romero JA, López M.

Center for Pharmaceutical Research and Drug Development (CIDEM), Ave 26 # 1605, Havana, Cuba. Phone number 8810882, 8811944, 8811424, Fax (537) 335556. E-mail: aylema.romero@cidem.sld.cu

 

Introduction: Tilipariti elatum S.W. (majagua) is considered an endemic specie of Cuba and Jamaica. Its flowers are prized by traditional medicine as expectorant and antiasthmatic. Rutin, quercetin and gossypitrin are the main flavonoids presents in the petals. Gossypitrin is the major component and it has been demonstrated its antioxidant, metal chelator and antimicrobial activity. Objective: The determination of optimal conditions of extraction with maximum yield of flavonoids, as markers from T. elatum, were determined by HPLC and total flavonoids using aluminin chloride method. Material and Methods: The extraction ratio (ratio of solvent to herbal drug), extraction time and extraction number were set as individual values and the yields of the metabolite were the response value that was optimized with a Box–Behnken design. Results: The optimal conditions obtained from response surface methodology (RSM) were 1:24.92 for the extraction ratio, 110.17 min for the extraction time and 3 for the extraction number. Under the optimal conditions, the response value of the experiment closely agreed with the predicted response value. Conclusions: The result suggests that RSM is successfully applied for optimizing the extraction of the marker compounds in T. elatum.

 

Citation Format: Barrios M, Romero JA, López M (2015) Optimization of extraction condition for flavonoids in Talipariti elatum S.W. Using response surface methodology. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S166. Abstract nr PACH-73.