J Pharm Pharmacogn Res 3(suppl. 1): S167, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
PACH-74: GAS CHROMATOGRAPHY DETERMINATION OF THE FATTY ACIDS CONCENTRATION DURING SUNFLOWER OIL OZONATION
Támbara Y1, Flores D2, Hernández K2, Álvarez A1, Fernández LA2, Gil D2, Ledea O2.
Introduction: The ozonized vegetable oils are products of natural origin that presents an increasing interest in the development of drugs, nutraceutics and cosmetics. The vegetable oils are composed by a mixture of triglycerides with different grades of unsaturation, depending of its components fatty acids. Through of its unsaturation, these compounds react with ozone. The aim of this work is the determination by gas chromatography of the fatty acids concentration during the ozonation reaction. In addition, a correlation of this determination with the peroxide value, usually employed in the physico- chemical characterization of this ozonized compounds is required. Material and Methods: The sunflower oil was ozonated in a burbling reactor at controlled temperature. The determination of the fatty acids concentration in the sunflower oil during the ozonation was making by gas chromatography analyzing its fatty acids methyl esters derivatives. In addition, samples were characterized by peroxide and acidic values, viscosity and UV-visible spectroscopy. Results: The composition of fatty acids found in the sunflower oil confirms the reported in the literature. The decreasing of the linoleic acid (C18:2) concentration followed by the oleic acid (C18:1), during the ozonation reaction of the oil, was also confirmed. The peroxide value increases with the unsaturated fatty acids reduction. Conclusions: The employed technique for the determination of the fatty acid concentration was found to be effective for following the ozone – sunflower oil reaction. A good correlation was found between the decreasing of the unsaturated fatty acids and the peroxide value, usually indicator used.
Citation Format: Támbara Y, Flores D, Hernández K, Álvarez A, Fernández LA, Gil D, Ledea O (2015) Gas chromatography determination of the fatty acids concentration during sunflower oil ozonation. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S167. Abstract nr PACH-74.