PACH-76: ANTITUMORAL ACTIVITY OF ETHANOLIC EXTRACT OF THE BARK OF Maytenus buxifolia SUBSP. cajalbanica

J Pharm Pharmacogn Res 3(suppl. 1): S169, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PACH-76: ANTITUMORAL ACTIVITY OF ETHANOLIC EXTRACT OF THE BARK OF Maytenus buxifolia SUBSP. cajalbanica (BOHIRDI; O.MUÑIZ). METABOLIC FINGERPRINT BY (FIA/ESI/MSN)

Pino L1, Rodeiro I2, Hernández I2, Spengler I1, García T1, Fernández A1, Vilegas W3, Pérez C1.

1Center for Natural Product Studies, Faculty of Chemistry, University of Havana, Cuba. E-mail: liu@fq.uh.cu
2CEBIMAR, Havana, Cuba
3Experimental Campus of Sao Vicente, UNESP—Sao Paulo State University, Sao Vicente, Sao Paulo, Brazil.

 

Introduction: Severals plants of the genus Maytenus, belonging to the family Celastraceae, are used in South America to prepare infusions or decoctions that are used as anti-inflammatory, analgesics, anti-ulcer remedies. Phytochemicals and biological studies of extracts from various species of the genus Maytenus demonstrated some anti-inflammatory, analgesic, diuretic, antitumor, antioxidant, antiulcer, aphrodisiac, among others properties. In addition, previous researches highlighted the existence of numerous secondary metabolites, which include flavonoids, catechins, galocatechins and their epi isomers, which sometimes are responsible for their activity. The species Maytenus buxifolia subsp. cajalbanica (Bohirdi; O.Muñiz) is endemic from Cuba and has not gone in to phytochemical and biological studies. In this work we set aim to evaluate the anti-tumoral activity of ethanolic extract of the bark of this endemic species and obtaining their metabolic fingerprint using direct flow injection electrospray ionization tandem mass spectrometry (FIA/ESI/MSn). Material and Methods: The dried ground plant material was extracted by soxhlet extraction using diethyl ether/petroleum ether 1:1 and subsequently with ethanol. Qualitative chemical composition of the extract was determined by direct flow injection electrospray ionization tandem mass spectrometry (FIA/ESI/MSn) in negative mode. Thermo Finnigan LCQ Deca ion trap mass spectrometer (San Jose, CA, USA) equipped with an ESI interface was used. The cells used in antitunoral study are acquired from the American type culture collection (ATCC). Results and Conclusions: Here we reported for the first time the antitumoral activity of ethanolic extract of the bark of this endemic species against the tumour cell lines 4T1 and EA.hy926. Monomers, dimers, trimmers and tetramers of proanthocyanidins were identified.

 

Citation Format: Pino L, Rodeiro I, Hernández I, Spengler I, García T, Fernández A, Vilegas W, Pérez C (2015) Antitumoral activity of ethanolic extract of the bark of Maytenus buxifolia SUBSP. cajalbanica (Bohirdi; O.Muñiz). Metabolic fingerprint by (FIA/ESI/MSN). [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S169. Abstract nr PACH-76.