PACH-84: PHYTOCHEMICAL EVALUATION AND ESTIMATION OF THE CONTENT OF POLYPHENOLS AND FLAVONOIDS IN EXTRACTS OF MULBERRY

J Pharm Pharmacogn Res 3(suppl. 1): S177, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PACH-84: PHYTOCHEMICAL EVALUATION AND ESTIMATION OF THE CONTENT OF POLYPHENOLS AND FLAVONOIDS IN HYDROALCOHOLIC EXTRACTS OF MULBERRY (MORERA) LEAVES HARVESTED IN CUBA

Almora E1, Lago V1, González K2, Callicó A1, Bolaños G2, Valdéz O2, Echemendía O1, Adames Y1, Monteagudo R1, Fidalgo O1, Morales Y1.

1Instituto Finlay, Playa, La Habana, Cuba. E-mail: ealmora@finlay.edu.cu
2Centro de Bioproductos Marinos (CEBIMAR), Plaza, La Habana, Cuba.

 

Introduction: Morus alba, commonly known as mulberry, is a tree belonging to the genus Morus, family Moraceae. It is used as an ornamental plant; its leaves are rich in minerals, vitamins, folic acid, amino acids, and it is the only food of the silkworm. It has several medicinal properties, which have been used in the treatment of acne, as an antipyretic, diuretic and reducing cholesterol and blood pressure. Recent studies have developed the antiproliferative capacity against cancer. Phytochemical characterization of plants is important because through these studies may suggest the presence of actives principles with biological activity such as flavonoids, polyphenols, among others. Objective: Phytochemical screening and determining the content of polyphenols and flavonoids in hydroalcoholic extracts of mulberry leaves. Material and Methods: The plant material was dried under controlled temperature and humidity. The screening was performed by classical fractionation methodology solvent polarity. Alcoholic extraction was performed at different concentrations (96, 70, 50 and 30%), at room temperature for three days and the polyphenol and flavonoid content was determined by standard techniques as described using pyrogallol and quercetin, respectively. Results: Oil/fat, alkaloids, triterpenes/steroids, catechins, reducing sugars, flavonoids and phenol/tannins were detecting. The highest polyphenol content was found in 95% ethanol extract (293.29 mg/g) and the lowest in extract 30% (52.74 mg/g). The flavonoids behaver was similarly to polyphenols, being the 95% ethanol extract the higher value (455.46 mg/g) and 30% the lower value (62.61 mg/g). Conclusions: Six groups of metabolites were observed. The highest polyphenols and flavonoids content were found in 95% ethanol extract.

 

Citation Format: Almora E, Lago V, González K, Callicó A, Bolaños G, Valdéz O, Echemendía O, Adames Y, Monteagudo R, Fidalgo O, Morales Y (2015) Phytochemical evaluation and estimation of the content of polyphenols and flavonoids in hydroalcoholic extracts of mulberry (morera) leaves harvested in Cuba. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S177. Abstract nr PACH-84.