PETM-67: PRECLINICAL VALIDATION OF TRADITIONAL USE OF Hibiscus rosa-sinensis

J Pharm Pharmacogn Res 3(suppl. 1): S159, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PETM-67: PRECLINICAL VALIDATION OF TRADITIONAL USE OF Hibiscus rosa-sinensis (MARPACÍFICO)

Brito G, Martínez I, Morón F, Victoria MC, Duménigo A, Morejón Z, Nossin E.

Central Laboratory of Pharmacology, Faculty of Medical Sciences. “Dr. Salvador Allende”. Carvajal e/ Agua Dulce and A, Cerro, CP 12000, Havana, Cuba. E-mail: gisellebrito@infomed.sld.cu

 

Introduction: In Cuba and Caribbean countries Hibiscus rosa-sinensis are very common in garden. Reports of traditional use are related with conjunctivitis, fever, headache, flu and cough. Our aim was to determinate preclinical anti-inflammatory, analgesic and antipyretic effect of decoction of leaves and flowers of H. rosa. Material and Methods: Activity was assayed on acute inflammation model (ear edema test), applying 10 µL croton oil, 75 µg/per ear. Different doses of decoctions 30% of flowers and leaves were evaluated topically and orally; 10 µL of decoctions were applied at each side of the treated ears. Model of analgesic was writhing test (acid acetic 0.75 %, 0.1 mL/10 g i.p) and tail flick test (water at 55°C). All experimental models was conducted in male OF-1 mice (20–25 g). Finally antipyretic activity was tested inducing pyrexia with brewer’s yeast (15% in water; 1 mL/100 g, sc.) in male Wistar rats (180-200g). Results: The extracts from leaves and flowers (30%) applied topically inhibited inflammation on 54.7% and 30.9% respectively. Orals administrations do not showed a significantly antiinflammatory activity in both extracts, but they have a significant anti-nociceptive response to thermal stimulus compared to control group. Decoctions of H. rosa significantly decreased the number of abdominal stretching in all cases. Only decoction of flowers showed a significant antipyretic activity similar to paracetamol, from the 1fts hour to 4 h after administration. Conclusions: Results validated traditional use of leaves or flowers of Hibiscus rosa sinensis in inflammation, flu, cough and pain process.

 

Citation Format: Brito G, Martínez I, Morón F, Victoria MC, Duménigo A, Morejón Z, Nossin E (2015) Preclinical validation of traditional use of Hibiscus rosa-sinensis (marpacífico). [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S159. Abstract nr PETM-67.