Philippines Equisetum hyemale as potential hair grower

J Pharm Pharmacogn Res 7(5): 323-330, 2019.

Original Article | Artículo Original

Hair regenerative activities of flavonoid-rich extract of Equisetum hyemale L. (Equisetaceae) in chemically-induced alopecia in Sprague Dawley rats

[Actividad regeneradora del cabello de extracto rico en flavonoides de Equisetum hyemale L. (Equisetaceae) en alopecia inducida químicamente en ratas Sprague Dawley]

Penuel P. David1,2*, Cecilia D. Santiago1,3, Anges L. Castillo4, Gerard Q. De Guzman1,3,5,6,7

1Graduate School, Centro Escolar University, Manila, Philippines.
2Department of Pharmacy, Centro Escolar University, Malolos, Philippines.
3School of Pharmacy, Centro Escolar University, Manila, Philippines.
4Faculty of Pharmacy and the Graduate School, University of Santo Tomas, Manila, Philippines.
5College of Pharmacy and Graduate School, Adamson University, Manila, Philippines.
6Francisco Q. Duque Medical Foundation, College of Medicine, Lyceum Northwestern University, Dagupan, Pangasinan, Philippines.
 7The Graduate School, Virgen Milagrosa University Foundation, San Carlos, Pangasinan, Philippines.
Abstract

Context: The prevalence of alopecia around the world is high and awareness continuously increased due to social stigma. There are limited commercially-available medications for the management of different forms of baldness, most of which are prohibitively expensive and presents with various adverse effects.

Aims: To evaluate the flavonoid-rich ethyl acetate extract of Equisetum hyemale stem for its hair-regenerative properties in chemically-induced alopecia in Sprague Dawley rats.

Methods: Various concentrations of the flavonoid-rich extract of E. hyemale were applied in depilated areas in dorsal rat skin after chemical induction of alopecia. Evidence of hair growth was observed for 28 days in a weekly basis. Histopathological analysis of rat skin was performed to demonstrate evidence of follicular hair growth.

Results: The abundance of flavonoids in the ethyl acetate extract was established by total flavonoid contents and LC-MS analyses. The 2.5% E. hyemale extract exhibited hair-regenerative properties with high tensile strengths, combined masses and follicular growth, which was comparable to the positive control, 5% minoxidil (p>0.05). The highest rate of hair follicular development was observed in the telogen phase in rat skin after 28 days of treatment.

Conclusions: The flavonoid-rich ethyl acetate extract of E. hyemale exhibits in situ hair-regenerative properties in chemically-induced alopecia in rats at 2.5% w/v concentration.

Keywords: alopecia; Equisetum hyemale; flavonoids; hair-regenerative; horsetail.

Resumen

Contexto: La prevalencia de alopecia en todo el mundo es alta y la conciencia aumenta continuamente debido al estigma social. Existen medicamentos disponibles pero limitados para el tratamiento de diferentes formas de calvicie, la mayoría de ellos son muy caros y presentan diversos efectos adversos.

Objetivos: Evaluar el extracto de acetato de etilo, rico en flavonoides, del tallo de Equisetum hyemale por sus propiedades regenerativas del cabello en la alopecia inducida químicamente en ratas Sprague Dawley.

Métodos: Se aplicaron diversas concentraciones del extracto rico en flavonoides de E. hyemale en áreas depiladas en la piel dorsal de rata después de la inducción de la alopecia. La evidencia de crecimiento del pelo se observó semanalmente durante 28 días. El análisis histopatológico de la piel de rata se realizó para demostrar la evidencia de crecimiento folicular del pelo.

Resultados: La abundancia de flavonoides en el extracto de acetato de etilo se estableció mediante el contenido total de estos y el análisis LC-MS. El extracto de E. hyemale al 2,5% mostró propiedades de regeneración del cabello con altas resistencias a la tracción, masas combinadas y crecimiento folicular, que fue comparable al control positivo, 5% de minoxidil (p> 0.05). La mayor tasa de desarrollo folicular del cabello se observó en la fase telógena en la piel de rata después de 28 días de tratamiento.

Conclusiones: El extracto de acetato de etilo, rico en flavonoides, de E. hyemale exhibe propiedades regenerativas del cabello in situ en la alopecia inducida en ratas a una concentración de 2,5% p/v.

Palabras Clave: alopecia; cola de caballo; Equisetum hyemale; flavonoides; regenerador del cabello.

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Citation Format: David PP, Santiago CD, Castillo AL, De Guzman GQ (2019) Hair regenerative activities of flavonoid-rich extract of Equisetum hyemale L. (Equisetaceae) in chemically-induced alopecia in Sprague Dawley rats. J Pharm Pharmacogn Res 7(5): 323–330.

© 2019 Journal of Pharmacy & Pharmacognosy Research (JPPRes)