PMO-69: MEMORY ENHANCING EFFECTS OF BM-21IN AGED AND ALUMINIUM INTOXICATED MICE

J Pharm Pharmacogn Res 3(suppl. 1): S162, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PMO-69: MEMORY ENHANCING EFFECTS OF BM-21, AN AQUEOUS-ETHANOLIC EXTRACT OF THE MARINE PLANT Thalassia testidinum, IN AGED AND ALUMINIUM INTOXICATED MICE

García T1, Morales RA1, Palmero A1, García N1, Valdés O1, Fernández G2, Pérez C2, Menéndez R1.

1Center of Marine Bioproducts (CEBIMAR), Neuropharmacology Departament, Havana, Cuba. E-mail: teidy@cebimar.cu
2Institute of Medicine “Victoria de Girón”, Havana, Cuba.

 

Introduction: Memory is one of the earliest cognitive functions that decline during normal and pathological aging and it has a devastating social and economic impact. Oxidative damage accumulation in macromo-lecules has been considered one of the causative mechanisms. Here we assessed the effects of oral admi-nistration of BM-21 on learning and memory, and on indicators of oxidative stress in mice. Material and Methods: After examining general motor activity by open field and rota rod test, reference spatial memory performance was assessed by Morris Water Maze. Laten-cy to find the escape platform (escape latency, EL) during the training sessions, time in the target quadrant in the probe trial and the number of platform crossing were measured as indicators of cognitive performance. Results: Al-exposed (50 mg/kg daily, 3 months) and aged mice (19-month-old) showed more difficulties in learning the task when compared to non-Al exposed and young mice, respectively. BM-21 given at 100 mg/kg/day for 3 months significantly enhanced cogni-tive performance by decreasing EL and by increasing the time spent in the target quadrant and the number of platform crossing. Besides, the extract significantly reduced malondialdehide (MDA) concentration and increased reduced glutathione (GSH) and superoxide dismutase activity (SOD) in brain homogenates of aged and Al-exposed mice. Micronucleus studies showed that BM-21 extract reduced the frequency of micronucleated-binucleated cells in aged mice bone marrow, which would be associated with its potent antioxidant properties.  In addition, the increase of AChE activity observed in Al-exposed group was reverted to normal by BM-21 administration. Conclusions: our results showed that sub-chronic administration of the extract (100 mg/kg) blocks age-dependent and Al-induced declined in spatial cognition in mice with a simultaneous reduction of oxidative stress in brain. It is thus possible that an antioxidant mechanism in brain will contribute to beneficial effects of BM-21 on memory lost.

Citation Format: García T, Morales RA, Palmero A, García N, Valdés O, Fernández G, Pérez C, Menéndez R (2015) Memory enhancing effects of bm-21, an aqueous-ethanolic extract of the marine plant Thalassia testidinum, in aged and aluminium intoxicated mice. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S162. Abstract nr PMO-69.