PNA-25: ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF PROTEOLYTIC FRACTION FROM Vasconcellea cundinamarcensis

J Pharm Pharmacogn Res 3(suppl. 1): S117, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-25: ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF PROTEOLYTIC FRACTION FROM Vasconcellea cundinamarcensis LATEX ON HAIRLESS MICE SKIN IRRADIATED WITH MULTIPLE DOSES OF UVB

Freitas KM1, Salas CE2, Lopes MTP1.

Departments of 1Pharmacology and 2Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. E-mail: cesbufmg@yahoo.com

 

Introduction: UV radiation induces skin damage by imbalance of the endogenous antioxidant system leading to an increase of free radical and inflammation. The P1G10 fraction from Vasconcellea cundinamarcensis latex, rich in cysteine protease, displays angiogenic, mitogenic and anti-inflammatory activities and enhances wound healing on different cutaneous lesion models. Here, we investigated the possible antioxidant and anti-inflammatory effects of topical P1G10 formulation against damage induced by multiples doses of UVB on mice skin. Methods: Hairless mice (n=49) were irradiated with UVB light (240 mJ/cm2/312 nm) on the dorsal area (protocol CETEA 174/2010). P1G10 (0.1-1.0% or vehicle – Natrosol®) was applied topically after daily UVB exposure for 10 days. One day after last treatment, the animals were anaesthetized, fragments of damaged skin were excised and analyzed for different markers. The catalase and GSH (Glutathione) activities were measured using spectrophotometric and fluorescent assays, respectively. Cytokines levels were determined by ELISA. The data were expressed as mean ± standard error and statistically analyzed by one way ANOVA, Newman-Keuls post-test. Results: The catalase activity was significantly enhanced by 0.1% or 0.5% P1G10 treatment (0.04 ± 0.003 vs 0.02 ± 0.003 ΔABS/µg protein/min – vehicle, p<0.05). Furthermore, a decrease in GSH, TNF-α and IL1β (p<0.05) levels was observed following each dose of P1G10. In addition, VEGF levels were reduced at 0.1% of P1G10 (2.70 ± 0.22 vs 3.06 ± 0.28 ρg/mg – vehicle, p<0.05). Conclusions:  The changes in catalase/GSH activities and cytokines levels suggest a role for P1G10 as antioxidant and anti-inflammatory, respectively, on tissue repair following UVB-induced damage. Financial Support: CNPq, FAPEMIG and CAPES.

 

Citation Format: Freitas KM, Salas CE, Lopes MTP (2015) Antioxidant and anti-inflammatory effects of proteolytic fraction from Vasconcellea cundinamarcensis latex on hairless mice skin irradiated with multiple doses of UVB. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S117. Abstract nr PNA-25.