PNA-26: In vitro AND in vivo ANTIOXIDANT EFFECT OF THE ETHANOLIC EXTRACT OF Solanum diploconos FLOWERS

J Pharm Pharmacogn Res 3(suppl. 1): S118, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-26: In vitro AND in vivo ANTIOXIDANT EFFECT OF THE ETHANOLIC EXTRACT OF Solanum diploconos FLOWERS

Venturi I1, Delgado-Roche L2, Menéndez R2, Morales RA2, Valdés O3, Hernández Y3, Malheiros A1, de Souza MM1, Cechinel-Filho V1, Rodeiro I2.

1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí, Santa Catarina, Brazil. E-mail: ivonilce.venturi@gmail.com
2Department of Pharmacology, 3Department of Chemistry Center of Marine Bioproducts, Havana, Cuba.

 

Introduction: Solanum diploconos is a species that belongs to the family of Solanaceae and the gender Solanum. The plant grows in Brazil, mainly in the regions of Santa Catarina. The scientific literature contains wide information on its botanic characteristics; however, little is known on the chemical composition and the biological properties of the plant. Thus, the aim of the present work was to evaluate the in vitro and in vivo antioxidant effect of the ethanolic extract of Solanum diploconos flowers. Material and Methods: The ethanolic extract was obtained by maceration and concentrated in a rotary evaporator at 40°C. The total content of flavonoids and phenolic compounds in the flowers extract was determined using standard procedures. The in vitro characterization of flowers included the ferric reducing power (FRAP), the inhibitory effect of brain phospholipid peroxidation, as well as the DPPH and superoxide anion scavenging capacity. Furthermore, a mouse model of benzo(a)pyrene (BaP)-induced oxidative stress was used to assess the in vivo antioxidant capacity of Solanum diploconos flowers. Results: The ethanolic flowers extract contains flavonoids (19.02 µg/g of dried extract) and phenolic compounds (3.14 mg⁄g of dried extract). The S. diploconos flowers inhibited (85.23%) the brain phospholipid oxidation (IC50=68.86 µg/mL), meanwhile the DPPH and superoxide anion scavenging capacity (IC50= 97.74 and 110.90 µg/mL, respectively) increased as the concentra-tion increases. The ferric reducing power was comparable with ascorbic acid. In addition, the oral administration of flower extract (10, 100 and 200 mg/kg) during a week prevented BaP-induced glutathione levels diminishment, together with a significant reduction (p<0.05) of lipid and protein damages. Conclusions: The present results demonstrated by the first time the in vitro and in vivo antioxidant capacity of Solanum diploconos flowers. Thus, the present work supports the potential use of this plant as a natural source of antioxidants to prevent oxidative stress-associated diseases.

Citation Format: Venturi I, Delgado-Roche L, Menéndez R, Morales RA, Valdés O, Hernández Y, Malheiros A, de Souza MM, Cechinel-Filho V, Rodeiro I (2015) In vitro and in vivo antioxidant effect of the ethanolic extract of Solanum diploconos flowers. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S118. Abstract nr PNA-26.