PNA-29: EVALUATION OF ANTIOXIDANT ACTIVITY AND ACUTE TOXICITY OF AQUEOUS FRACTION OF Dichrostachys cinerea LEAVES

J Pharm Pharmacogn Res 3(suppl. 1): S121, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-29: EVALUATION OF ANTIOXIDANT ACTIVITY AND ACUTE TOXICITY OF AQUEOUS FRACTION OF Dichrostachys cinerea (L.) WIGHT & ARN. (Mimosaceae) LEAVES

Ribalta V1, Torres L2, Hernández E1, Sueiro M1, Armas Y1, Ruz V1.

1Pharmacy Department, Chemistry and Pharmacy Faculty. Central University of Las Villas, Santa Clara, Cuba. Road to Camajuaní km 5 ½. e-mail: venancior@uclv.edu.cu. Phone:42- 222544.
2Pharmacy Department, Food and Pharmacy Institute. University of Havana. Havana, Cuba.

 

Introduction: The aim of this study was to evaluate the in vitro antioxidant properties and the acute oral toxicity of the aqueous fraction of the D. cinerea leaves (AFDC) in Sprague-Dawley rats. Material and Methods: Plant material: Leaves were collected in the Botanical Garden of the university. Plant sample was identified as Dichrostachys cinerea by a taxonomic expert of the above institution. Phytochemical procedure: Dry powder (30 g) was extracted with methanol using Soxhlet apparatus. Methanol was removed under reduced pressure at 40°C to obtain a residue. It was suspended in water and partitioned with n-hexane to degrease. AFDC was collected and store at 2-8 °C until use. Antioxidant properties of D. cinerea: The ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl dihydrazyl (DPPH) and Fe2+-chelating assays were used. The total phenolic (TPC) and total flavonoids contents (TFC) were estimated by Folin-Ciocalteu and AlCl3 methods respectively, using galic acid (GA) and quercetin (Q) as standards. Acute Oral Toxicity: Test was carried out according to the single doses method on Sprague-Dawley rats at 2000 mg/kg body weight. Results and Discussion: Positive results were found for flavonoids, tannins and saponins. Antioxidant activity: AFDC had effective antioxidant properties giving IC50 values of 5.83 ± 0.03 µg/mL   by ferric ions (Fe3+)- reducing power, 3.00 ± 0.00 µg/mL by DPPH assay and 8.28 ± 1.04 µg/mL by ferrous ions (Fe 2+) chelating activity. The total phenolic content (TPC) and total flavonoids content (TFC) were 243 ± 8.71 µg GAE/mg and 20 ± 0.48 µg QE/mg, respectively. Acute Toxicity: There was no mortality or behavioral abnormality in rats treated orally with a dose of 2000 mg/kg body weight. Conclusions: The results obtained suggest that the aqueous fraction of D. cinerea leaves possess significant in vitro antioxidant activity and can be considered as safe up to 2000 mg/kg body weight.

 

Citation Format: Ribalta V, Torres L, Hernández E, Sueiro M, Armas Y, Ruz V (2015) Evaluation of antioxidant activity and acute toxicity of aqueous fraction of Dichrostachys cinerea (L.) Wight & Arn. (Mimosaceae) leaves. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S121. Abstract nr PNA-29.