PNAI-30: In vitro ANTILEISHMANIAL ACTIVITY OF Heliotropium indicum LINN AND Cordia dentata POIR FRACTIONS

J Pharm Pharmacogn Res 3(suppl. 1): S122, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-30: In vitro ANTILEISHMANIAL ACTIVITY OF Heliotropium indicum LINN AND Cordia dentata POIR FRACTIONS

Gómez-Estrada H1, Mercado-Camargo J1, Cervantes-Ceballos L1, Jiménez-Villalobos T1, Robledo-Restrepo S2.

1Grupo de Investigación Química de Medicamentos. Facultad Ciencias Farmacéuticas. Universidad de Cartagena. Cartagena-Colombia. E-mail: hgomeze@unicartagena.edu.co
2Programa de Estudio y Control de Enfermedades Tropicales PECET. Universidad de Antioquia, Medellín-Colombia.

 

Introduction: Medicinal plants play an important role between different biological processes as antitumor, cytotoxicity, antimicrobial, anti-inflammatory, anti-estrogen, anti-allergic, antioxidant, among other. Colombia has about 10% of all plant species in the world, being the North coast with highest medicinal herbs few studied. This study aims to evaluate in vitro cytotoxic activity on human promonocytic cell line U937 and leishmanicidal activity against intracellular amastigotes of Leishmania (V) panamensis (MHOM/CO/87/UA140-EpiR-GFP strain) of fractions obtained from leaves Heliotropium indicum Linn and Cordia dentata Poir. Material and Methods: The plants were collected in 2013 at the North of Bolivar (Colombia). The cytotoxic activity was performed using 2,5-diphenyl-tetrazolium enzymatic method 3-[4,5-dimethylthiazol-2-yl] (MTT) and antileishmanial assessment by flow cytometric technique. The assays were done by triplicate in at least two independent experiments, as positive control was used amphotericin B. Results were expressed as fifty cytotoxic concentrations (CC50), concentration necessary to kill 50% of cells, and fifty effective concentrations (EC50) calculated by Probit analysis (Parametric method of linear regression that permits doses-response analysis); it was similarly determined the Selectivity Index (SI). Results: H. indicum (Hi) fractions were more cytotoxic than C. dentata (Cd) against cell line U937. Hi-I- 18B and Hi-I-18E fractions were considered as potentially cytotoxic (CC50 11.7 and 13.9 mg/mL). Moreover, the Cd-I-13C fraction was midly cytotoxic (CC50 139.1 µg/mL). The fractions were first screened for antileishmanial activity against Leishmania (V) panamensis at 13. 5 ± 0.3 mg/mL, IS = 0.9 (Hi-I- 18B) and 11.5 ± 2.6 mg/mL, IS = 1.2 (Hi-I-18E) showed % growth inhibition were 18.5 and 21.8%. Cd-I-13C at 24.5 mg/mL, IS = 5.7, growth inhibition was 54.3%. Conclusions: This suggests that plants species are rich potential source of molecules useful for treatment of cancer and parasitic diseases. Financial Support: COLCIENCIAS, Colombia (Project 1107-569-33684/512-2012)

Citation Format: Gómez-Estrada H, Mercado-Camargo J, Cervantes-Ceballos L, Jiménez-Villalobos T, Robledo-Restrepo S (2015) In vitro antileishmanial activity of Heliotropium indicum Linn and Cordia dentata Poir fractions. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S122. Abstract nr PNAI-30.