PNAI-32: ANTIBACTERIAL EFFECT OF Costus spiralis LEAVES EXTRACT ON PATHOGENIC STRAINS OF Vibrio cholera

J Pharm Pharmacogn Res 3(suppl. 1): S124, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-32: ANTIBACTERIAL EFFECT OF Costus spiralis LEAVES EXTRACT ON PATHOGENIC STRAINS OF Vibrio cholera

Pérez C, Falero A, Hung BR, Ledón T, Fando R.

Centro Nacional de Investigaciones Científicas, Ave 25 Esq 158, Playa. La Habana, Cuba. E-mail: talena.ledon@cnic.edu.cu

 

Introduction: The use of remedies from plant origin covers a wide variety of maladies and constitutes an alternative way to antibiotic therapy, which otherwise seems to be no longer promising due to antibiotics widespread resistance among pathogenic microorganisms. Active principles having antimicrobial activity may be extracted and purified from plants for developing new drugs. Among several illnesses that have historically scourged man, cholera has been potentially epidemic and one of the most outstanding. The bacterium Vibrio cholerae, the causal agent, can be eliminated with antibiotics so that besides the traditional treatment of rehydration via oral or intravenous, antibiotics such as tetracycline, ciprofloxacin, norfloxacin or azithromycin are commonly applied. Material and Methods: The antimicrobial effect of Costus spiralis (Roscoe) leaves extracts on various pathogenic strains of Vibrio cholerae was assayed in vitro by means of the agar plate diffusion technique. Fresh leaves from this plant were picked up, ovendried for 48 h at 50°C, powdered and finally ethanol-extracted. After drying, the remaining residue was suspended in distilled water up to 100 mg/mL (w/v) and the antimicrobial assays carried out. The pathogenic strains representing the pandemics of the 20th Century: C7258 (O1, El Tor, Ogawa), C6706 (O1, El Tor, Inaba), O395 (O1, Classic, Ogawa), CRC266 (O139) and 569B (O1, Classic, Inaba) were apparently killed, as judged by halos of inhibition of growth in the assays. Similar minimal inhibitory concentrations (MICs) of the extracts for the various strains were obtained. The above results were similar to those from ampicillin. Results and Conclusions: Costus spiralis may be used as a source of active principles against Vibrio cholera and may be considered as an alternative way to antibiotic therapy for cholera, mostly in those cases where the disease becomes epidemic and sanitary conditions were not promptly available.

 

Citation Format: Pérez C, Falero A, Hung BR, Ledón T, Fando R (2015) Antibacterial effect of Costus spiralis leaves extract on pathogenic strains of Vibrio cholera. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S124. Abstract nr PNAI-32.