J Pharm Pharmacogn Res 3(suppl. 1): S125, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
PNAI-33: CYTOSTATIC AND CYTOTOXIC EVALUATION OF EXTRACTS FROM FIVE CUBAN PLANTS WITH POSSIBLE ANTIMICROBIAL, ANTIPARASITIC AND ANTI-PROLIFERATIVE EFFECT
Mancebo B, Regalado AI, Sánchez LM.
Introduction: The inhibition of seed germination and Artemia salina lethality test are considered a useful tool for the preliminary toxicity determination of plant extracts. The objective of the present work was to determine the cytostatic and cytotoxic effect of total methanol extracts from leaves and stems obtained from the species Ta, Th, Gl, Va and Cc belonging to Bignonacea family; and hexane, ethyl acetate and methanol fractions. Material and Methods: The cytostatic effect was determined by the seed germination inhibition test of Solanum lycopersicum (tomato) and Lactuca sativa (lettuce) of total methanol extracts and fractions of the species under study. Cytotoxicity was performed by Artemia salina lethality test. Results: From 10 of the total methanol extracts studied, four showed cytostatic effect at the tested concentrations, where Gl (leaves) extract showed the best effect. Moreover, of the 15 fractions studied, Gl (leaves) acetate, Th (leaves) acetate and methanol and Cc (leaves) acetate, showed promising cytostatic effect, where Th (leaves) acetate fraction showed the highest activity. As regards toxicity study with brine shrimp larvae, all total methanol extracts showed moderate activity, however Gl (leaves), Ta (leaves) and Th (leaves) fractions were classified as highly toxic. Conlusions: The cytostatic and cytotoxic activities of the species studied are showed by the results of this research, constituting the first report of the same, which could have active substances associated with promising antimicrobial, antiproliferative and antiparasitic activity.
Citation Format: Mancebo B, Regalado AI, Sánchez LM (2015) Cytostatic and cytotoxic evaluation of extracts from five Cuban plants with possible antimicrobial, antiparasitic and anti-proliferative effect. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S125. Abstract nr PNAI-33.