PNAI-37: In vitro ANTIFUNGAL ACTIVITY OF CRUDE HYDRO-ALCOHOLIC EXTRACT OF Petiveria alliacea L. ON CLINICAL Candida ISOLATES

J Pharm Pharmacogn Res 3(suppl. 1): S129, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-37: In vitro ANTIFUNGAL ACTIVITY OF CRUDE HYDRO-ALCOHOLIC EXTRACT OF Petiveria alliacea L. ON CLINICAL Candida ISOLATES

Velar RE1, Illnait MT1, Illnait J2, Fernández CM1, Martínez GF1, Perurena MR1, Monroy EX1, Meis JF3.

1Mycology Laboratory; Tropical Medicine Institute “Pedro Kourí” (IPK). Apartado Postal 601, Marianao 13, La Lisa. Havana, Cuba. Phone: 53-7-255 3524. Fax: 53-7-336048. E-mail: velar@ipk.sld.cu
2Natural Products Laboratory, National Centre for Scientific Research (CNIC). Ave. 25 and 158 Street, Apartado Postal 6412, Playa. Havana, Cuba. E-mail: jose.illnait@cnic.edu.cu
3Regional Public Health Laboratory Service. Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital (CWZ), Weg door Jonkerbos 100 6532 SZ Nijmegen. P.O. Box 9015 6500 GS Nijmegen, The Netherlands. Phone: 31 243657514. E-mail: j.meis@cwz.nl

 

Introduction: The global burden of infections due to Candida and the emerging resistance to antifungals has led to the search for new therapeutic alternatives. The aim of the present work was to evaluate the in vitro antifungal activity of crude hydro-alcoholic extract of Petiveria alliacea L. (HAEPAL) versus fluconazole against Candida isolates. Methods: In vitro antifungal activity was evaluated by broth microdilution method in front of 125 Candida isolates (60 C. albicans). Inoculum of 1.5 x 106 CFU/mL in sterile saline solution was incubated with five dilutions of the extract (128, 64, 32, 16 and 8 μg/mL). The minimum inhibitory concentration (MIC) was defined as the lowest concentration of HAEPAL showing ≤50% growth compared with the extract-free growth control estimated by counting the CFU/mL. Fluconazole susceptibility was assessed by ATBTM Fungus 3 and E-test according to manufacturer’s instructions. Results: HAEPAL showed higher antifungal activity compared to fluconazole. Only four isolates (one C. albicans, one C. glabrata and two C. krusei) exhibited high MICs (≥ 64 μg/mL) compared to 34 (19 C. albicans and 15 Candida non-albicans), which were resistant to fluconazole. Conclusions: These results show the antifungal potentiality of HAEPAL, which could become a potential alternative for Candida treatment.

 

Citation Format: Velar RE, Illnait MT, Illnait J, Fernández CM, Martínez GF, Perurena MR, Monroy EX, Meis JF (2015) In vitro antifungal activity of crude hydro-alcoholic extract of Petiveria alliacea L. on clinical Candida isolates. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S129. Abstract nr PNAI-37.