PNAI-38: ANTIPLASMODIAL ACTIVITY AND ACUTE ORAL TOXICITY OF HYDROALCOHOLIC EXTRACTS OF Zuelania guidonia

J Pharm Pharmacogn Res 3(suppl. 1): S130, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-38: ANTIPLASMODIAL ACTIVITY AND ACUTE ORAL TOXICITY OF HYDROALCOHOLIC EXTRACTS OF Zuelania guidonia

Puente E1, Mora C2, Jaramillo M2, Berenguer C1, Rodríguez E1, Salas I1, Reinert A1, Cedeño N1.

1Centro de Toxicología y Biomedicina (TOXIMED), Autopista Nacional Km. 1 ½. Apartado: 4033, Santiago de Cuba. Código Postal: 90400. Cuba. E-mail: zapata.puente@medired.scu.sld.cu
2Facultad de Química Farmacéutica, Universidad de Antioquia, Colombia.

 

Introduction: According to estimates by the World Health Organization (WHO), malaria is a disease caused by parasites of the Plasmodium family. In 2013 it produced 198 million cases, which caused the death of about 584 000 people. The malaria mortality rate has dropped by over 47% since 2000 worldwide. A There is no licensed vaccine against malaria or other human parasite. Ethnobotanical studies showed data on the Zuelania guidonia and antiparasitic activity in areas of Cuba and Central America. Considering the biological evaluation performed raised two hydroalcoholic extracts vs Plasmodium, in vitro plant and acute oral toxicity thereof. Material and Methods: Extracts were obtained by the Soxhlet method, using as raw material to extract one leaves and bark extract two, a hydroalcoholic at 60 and 70 respectively menses. The antimalarial activity was performed by the method of continuous in vitro culture developed by Trager and Jensen (1976), chloroquine diphosphate (CQ) was used as positive control. To evaluate acute oral toxicity class method was used, following the rules of the OECD # 423 always starting dose of 300 mg/kg. Results: Evaluation in malaria Extract 1 was the active, with more than 45% of inhibition; the extract 2 had more than 18% inhibition. In acute oral toxicity they showed mortality rate of 300 < LD50 >2000 mg/kg. Conclusions: Thus it reaffirms that the formulations of the present antiplasmodial Zuelania guidonia toxicological activity and classified as dangerous in the animal model, dose levels employed and under the experimental conditions.

 

Citation Format: Puente E, Mora C, Jaramillo M, Berenguer C, Rodríguez E, Salas I, Reinert A, Cedeño N (2015) Antiplasmodial activity and acute oral toxicity of hydroalcoholic extracts of Zuelania guidonia. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S130. Abstract nr PNAI-38.