PPP-07: In vivo ANTI-INFLAMMATORY ACTIVITY OF EXTRACTS, FRACTIONS AND ISOLATED COMPOUNDS OF Tabebuia spp.

J Pharm Pharmacogn Res 3(suppl. 1): S99, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PPP-07: In vivo ANTI-INFLAMMATORY ACTIVITY OF EXTRACTS, FRACTIONS AND ISOLATED COMPOUNDS OF Tabebuia spp.

Regalado AI1, Sánchez LM1, Mancebo B1, Flogio MA2.

1National Center for Animal and Plant Health (CENSA), PO Box 10, ZC 32700, San José de las Lajas, Mayabeque, Cuba. E-mail: adi@censa.edu.cu, luzmaria@censa.edu.cu, betty@censa.edu.cu
2Centro Pluridisciplinar de Pesquisas Químicas Biológicas e Agrícolas (CPQBA), Campinas. Brasil. E-mail: foglioma@cpqba.unicamp.br

 

Introduction: Inflammation is a defense mechanism of the body and occurs against harmful stimuli; however, the extension may cause damage to cells and tissues. Chronic inflammatory diseases are a major health problem due to the lack of effective and safe drugs for use for long periods; this has augmented the investigations to provide new safer anti-inflammatory agents. Throughout history, nature, especially plants, has provided a source of medicines for the treatment of a wide spectrum of diseases. Tabebuia spp. is an endemic plant of Cuba, unexplored from the standpoint chemist-pharmacologic. Material and Methods: This research was conducted in order to assess the possible anti-inflammatory effect of this species using the carrageenin-induced paw edema models and the croton oil induced auricular edema in mice. Results: The methanolic extract of the leaves has no anti-inflammatory effect in the dose evaluated, whereas the methanolic extract of the stems showed an anti-inflammatory activity at dose of 500 mg/kg relative to the negative control group. Subsequently, the fractionation of the methanolic extract of the stems and pharmacological evaluation of these fractions is conducted, where ethyl acetate-methanol fraction was the most active. Moreover, it was possible to isolate a compound in ether-petroleum ethyl acetate fraction, it proved to be the majority in the stems, with an anti-inflammatory activity similar to indomethacin used as a positive control. Conclusions: The results of this research demonstrate the anti-inflammatory activity from Tabebuia spp stems, constituting the first preclinical report of anti-inflammatory effect in the species. Tabebuia spp. and their constituents could represent in the future a new therapeutic option for the treatment of inflammatory diseases.

 

Citation Format: Regalado AI, Sánchez LM, Mancebo B, Flogio MA (2015) In vivo anti-inflammatory activity of extracts, fractions and isolated compounds of Tabebuia spp. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S99. Abstract nr PPP-07.