PPP-21: CHEMOSENSITIZER EFFECT OF MICRO-ENCAPSULATED SEED LIPID EXTRACT OF Cucurbita pepo L. IN CT26.WT TUMOR CELLS

J Pharm Pharmacogn Res 3(suppl. 1): S113, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PPP-21: CHEMOSENSITIZER EFFECT OF MICRO-ENCAPSULATED SEED LIPID EXTRACT OF Cucurbita pepo L. IN CT26.WT TUMOR CELLS

Rodríguez-González JC, Quiñones-Maza OL, Valentín-Quiñones N, Nogueira-Mendoza A, Delgado-Hernández R.

Center for Pharmaceutical Research and Drug Development (CIDEM), Ave.26 No. 1605, entre Puentes Grandes y Boyeros, Nuevo Vedado, CP 10600, Plaza, Havana, Cuba. E-mail: jcesar.rodriguez@cidem.sld.cu, olgaq@infomed.sld.cu, rdelgado@infomed.sld.cu

 

Introduction: Decreasing side effects and chemo-resistance and increasing specific tumor targeting is the greatest challenge in cancerology research. Natural compounds have been identified that enhance the effectiveness of conventional chemotherapy and radiotherapy. The chemical components of seed lipid extract from Cucurbita pepo L. have antitumor and chemosensitizer adjuvant activities; however, the use of seed lipid extract as phytochemical chemosensitizer adjuvant in anticancer combination therapy has not been reported yet. Objective: To evaluate the chemo-sensitizer adjuvant effect in vitro of microencapsulated seed lipid extract of C. pepo in combination treatments with antineoplastic platinum and 5-fluorouracil cytostatic agents. Methods: CT26.WT cell line was treated with the extract (1-200 μg/mL), cisplatin, oxaliplatin, carboplatin and 5-fluorouracil in simple and combination (sequential and simultaneous) treatments at different incubation times and at non cytotoxic concentrations. The cell survival was evaluated by MTT assay. Results: The extract was not cytotoxic and CT26.WT cells showed different sensibilities to anti-neoplastic agents (IC50: 5-FU = 0.457 μM, oxaliplatin = 1.009 μM, cisplatin = 2.625 μM, carboplatin = 26.07 μM). The results from combination treatments showed a general trend that the highest increases in cell death were obtained at higher concentrations of the extract (100/200 μg/mL) and the lowest concentration of antineoplastic agents (0,1 μM 5-fluorouracil, 1 μM oxali-platin and cisplatin, 2 μM carboplatin). In relation to treatment schemes, the best increases of cell death is achieved by simultaneous combination treatment in the cases of 5-fluorouracil, oxaliplatin and carboplatin; while presensitization sequential treatment of the cells with the extract was the best for cisplatin. The best of all chemosensibilizer effect was achieved in simultaneous scheme with oxaliplatin. Conclusions: Microencapsula-ted seed lipid extract of Cucurbita pepo L. in combination treatment with cytostatic platinum and 5-fluorouracil potentiates cytostatic-induced cell death in our experimental conditions. Financial Support: Project of Cuban Ministry of Health nr. 1401049.

Citation Format: Rodríguez-González JC, Quiñones-Maza OL, Valentín-Quiñones N, Nogueira-Mendoza A, Delgado-Hernández R (2015) Chemosensitizer effect of microencapsulated seed lipid extract of Cucurbita pepo L. in CT26.WT tumor cells. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S113. Abstract nr PPP-21.