PTS-39: CHRONIC ORAL TOXICITY OF TANNIN POWDERS OBTAINED FROM BARK OF Pinus caribaea MORELET IN RATS

J Pharm Pharmacogn Res 3(suppl. 1): S131, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PTS-39: CHRONIC ORAL TOXICITY OF TANNIN POWDERS OBTAINED FROM BARK OF Pinus caribaea MORELET IN RATS

Curbelo A1, Bada AM1, Santana JL2, González B1, González Y1, González C1, Arteaga ME1, Gómez D1, Mancebo A1.

1Centro Nacional para la Producción de Animales de Laboratorio (CENPALAB), La Habana, Cuba. E-mail: ailemys.curbelo@cenpalab.inf.cu
2Instituto Superior de Ciencias y Tecnologías Aplicadas (InsTEC), La Habana, Cuba.

 

Introduction: Effects of antimutagenic and non-genotoxic agents of tannins from Pinus caribaea Morelet, as well as its influence in improvement of quality life of patients have been demonstrated. Our aim was to assess chronic oral toxicity of tannin powder obtained from bark of P. caribaea in Cenp:SPRD rats. Material and Methods: Rats were randomly distributed into four experimental groups: Control, Low (1 mg/kg), Median (2 mg/kg) and High Dose (5 mg/kg). Administration was made daily along the six months of the study. Rats were inspected daily for clinical signs. Body weight and food and water consumption were weekly measured. Hematological and biochemical analysis were performed at the beginning, three months and at the end of experimentation. Gross necropsy and histological examination of tissues were performed on animals at the end of the assay. Results: During the study a female in Low Dose group died, the remainder finished the study in a good state. Clinical signs described were distributed by all groups and were considered not to have relation with the tested substance. Body weight curve was similar for all groups. Water consumption increased in animals treated with the higher dose, but the food consumption was normal. Clinical laboratory parameters had differences, although they were within normal ranks reported for the species. There were no macroscopic or microscopic lesions related with the tested substance. Conclusions: The tannin powder obtained from bark of P. caribaea appears to be safe under the experimental conditions established.

 

Citation Format: Curbelo A, Bada AM, Santana JL, González B, González Y, González C, Arteaga ME, Gómez D, Mancebo A (2015) Chronic oral toxicity of tannin powders obtained from bark of Pinus caribaea Morelet in rats. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S131. Abstract nr PTS-39.