PTS-41: STABILITY AND TOXICITY OF LEAF EXTRACT OF Spondias mombin L.

J Pharm Pharmacogn Res 3(suppl. 1): S133, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PTS-41: STABILITY AND TOXICITY OF LEAF EXTRACT OF Spondias mombin L.

Pérez-Portero Y1, Losada-Martínez L1, Bignott-Tissert Y1, Rodríguez-Leblanch E3, Cedeño-Soularit N3, Mourlot-López A3, González-Giro Z3, Escalona-Arranz J2.

1Department of Biology, Faculty of Natural Science, Universidad de Oriente. Patricio Lumumba s/n CP 90 500, Santiago de Cuba, Cuba. E-mail: yalinapn@cnt.uo.edu.cu
2Department of Pharmacy, Faculty of Natural Science, Universidad de Oriente, Santiago de Cuba, Cuba.
3Centro de Toxicología y Biomedicina (TOXIMED), Santiago de Cuba, Cuba.

 

Introduction: Spondias mombin L. is a species used for the treatment of various infectious diseases. In this research was determined the physical-chemical and microbiological stability of a 70% alcoholic extract from leaves of Spondias mombin L. Material and Methods: For this purpose, the samples of (EHSM) were analyzed during 360 days and the oral acute toxicity in one dose was determined by performing the CTA method. Samples were collected at “El Caney” region, Santiago de Cuba; 50 g of dried leaves by oven were manually crushed and then, 150 mL of alcohol-water solvent (70:30) were added; after that procedure, maceration was performed for 48 h and subsequently rotoevaporated to 50 mL. To determine the physical-chemical stability these parameters were measured: relative density, total dissolved solids, pH, relative viscosity and electrical conductivity, established by the current Cuban Norm (NRSP 312, 1991) and, for the microbiological stability, the Cuban Norm 68-2007 was followed; parameters were determined at 0, 7, 30, 90, 180 and 360 days. The acute oral toxicity test based on the class method was conducted in Sprague Dawley rats. Results and Conclusions: The EHSM showed physical-chemical and microbiological stability. This extract did not show any clinical signs of toxicity or animal deaths and orally administered at one dose is regarded as unclassified for the animal model and the dosage used under the observed experimental conditions, according to the specific standard of the Organization for Economic Cooperation and Development (OECD 423: 2001).

 

Citation Format: Pérez-Portero Y, Losada-Martínez L, Bignott-Tissert Y, Rodríguez-Leblanch E, Cedeño-Soularit N, Mourlot-López A, González-Giro Z, Escalona-Arranz J (2015) Stability and toxicity of leaf extract of Spondias mombin L. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S133. Abstract nr PTS-41.