PTS-45: TOXICITY EVALUATION AT SINGLE DOSE AND DIURETIC ACTIVITY OF THE HYDROALCOHOLIC EXTRACT Mosiera bullata LEAVES

J Pharm Pharmacogn Res 3(suppl. 1): S137, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PTS-45: TOXICITY EVALUATION AT SINGLE DOSE AND DIURETIC ACTIVITY OF THE HYDROALCOHOLIC EXTRACT Mosiera bullata (BRITTON & P. WILSON) BISSE LEAVES

Armas-González Y, Sueiro-Oyarzun ML, Vicet-Muro L, Ribalta-Ribalta V, Hernández-Barreto E, Siverio-Mota D, Ruz-San Juan V, Valdés-Fuentes M.

Pharmacy Department, Chemistry and Pharmacy Faculty, Central University of Las Villas, Santa Clara, Villa Clara, Cuba. Road to Camajuaní km 5 ½. E-mail: yagonzalez@uclv.edu.cu

 

Introduction: Mosiera bullata (Britton & P. Wilson) Bisse is an endemic plant of the central region of Cuba, but its use is unknown. Previous phytochemical studies suggest potentialities for the therapeutic use. The objective of this study was to evaluate the acute toxicity at single dose and the possible diuretic effect of the hydroalcoholic extract from the leaves of the plant. Material and Methods: Acute toxicity test was carried out using the single doses method, where the extract was administered at 2000 mg/kg body weight to Sprague-Dawley rats. Diuretic activity of the extract (400 mg/kg) was conducted following the Lipschitz protocol. Male rats of the Wistar line were used in a weight range from 170 to 210 g, using sodium chloride (0.9%) as negative control and furosemide (20 mg/kg) and hydrochlorothiazide (10 mg/kg) as positive control. Concentration of sodium and potassium ions from the excreted urine and pH were also evaluated. Results: Normal behavior in animals, normal postural reflex, toilet habits and habitual response to nociceptive stimuli as well as food and water consumption were observed according to the specie. No sign of apparent toxicity nor death was observed. It was observed an increment in the volume of the urine The diuretic activity in the groups had an increment of the excreted urine, overcoming the excretion caused in the group negative control (chloride of sodium). The experimental group treated with the extract showed similar results to the volumes excreted by the group treated with hydrochlorothiazide, without significant statistical differences (p>0.05). Conclusions: The hydroalcoholic extract of Mosiera bullata (Britton & P. Wilson) Bisse is non toxic when the toxicity was evaluated at 2000 mg/kg. For the diuretic effect, the ratio Na+/K+ shows values that can be comparable with the thiazide diuretic.

 

Citation Format: Armas-González Y, Sueiro-Oyarzun ML, Vicet-Muro L, Ribalta-Ribalta V, Hernández-Barreto E, Siverio-Mota D, Ruz-San Juan V, Valdés-Fuentes M (2015) Toxicity evaluation at single dose and diuretic activity of the hydroalcoholic extract Mosiera bullata (Britton & P. Wilson) Bisse leaves. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S137. Abstract nr PTS-45.