Thalassia testudinum prevents acrylamide-induced neurotoxicity

J Pharm Pharmacogn Res 2(3): 53-62, 2014.

Original Article | Artículo Original

Neuroprotective and antioxidant effects of Thalassia testudinum
extract BM-21, against acrylamide-induced neurotoxicity in mice.

[Efectos neuroprotectores y antioxidantes del BM-21 extracto obtenido de Thalassia testudinum, sobre la neurotoxicidad inducida por acrilamida en ratones]

Roberto Menéndeza#*, Teidy Garcíaa#, Anoland Garateixa, Ruth A. Moralesa, Erik L. Regaladob, Abilio Lagunab, Olga Valdésb, Miguel D. Fernándeza

aDepartment of Pharmacology; bDepartment of Analytical Chemistry, Center of Marine Bioproducts (CEBIMAR). Calle Loma entre 35 y 37, Alturas del Vedado, Plaza de la Revolución, La Habana, CP. 10400, Cuba. #These authors equally contributed to this work.
*E-mails: roberto.menendez@infomed.sld.cu, rmenendezsoto@gmail.com
Abstract

Context: Acrylamide (ACR) neurotoxicity is associated with the enhancement of lipid peroxidation and the reduction of the antioxidative capacity distal axon and nerve terminal regions. The aqueous ethanolic extract of the marine plant Thalassia testudinum, named BM-21, have shown antioxidant, anti-inflammatory and analgesic properties.

Aims: To determine the neuroprotective and the antioxidant effects of BM-21, standardized to thalassiolin B content (5.8 ± 0.9%), on acrylamide (ACR)-induced distal axonopathy in male OF-1 mice.

Methods: Animals were administered with ACR (70 mg/kg, s.c., 4 weeks), and BM-21 was co-administered p.o at the doses of 4, 40 and 400 mg/kg. The effect of BM-21 on neurobehavioral indexes (rota-rod test), compound muscle action potential (CMAP) of the sciatic nerve and oxidative stress parameters were investigated.

Results: BM-21 significantly prevented the neurobehavioral sings of neurotoxicity and the alteration of CMAP amplitude and velocity. The lowest dose (4 mg/kg) failed to ameliorate these parameters whereas the highest dose (400 mg/kg) was the most active. BM-21 (400 mg/kg) significantly restored total hydroperoxides (THP) and glutathione (GSH) in the sciatic nerve as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities. Additionally, the extract also modified THP, GSH and the activity of SOD in cerebellum and brain towards the standard values.

Conclusions: BM-21 given at doses that prevented ACR-induced neurotoxicity also produced antioxidant effect in the sciatic nerve, cerebellum and brain. Thus, the neuroprotective activity of BM-21 in this model seems to be mediated at least partly by its antioxidative properties.

Keywords: Acrylamide-induced  axonopathy; oxidative stress; BM-21; Thalassia testudinum.

Resumen

Contexto: La neurotoxicidad inducida por acrilamida (ACR) se asocia al incremento de la peroxidación lipídica (POL) y a la reducción de la capacidad antioxidante de zonas distales de axón y terminales nerviosas. El extracto hidroetanólico de la planta marina Thalassia testididum (BM-21) posee efectos antioxidantes, anti-inflamatorios y analgésicos.

Objetivos: Determinar los efectos neuroprotectores y antioxidantes del BM-21 estandarizado acorde al contenido de thalassiolina B (5,8 ± 0,9%), sobre la axonopatía distal inducida por ACR en ratones OF-1.

Métodos: Los animales fueron administrados con ACR (70 mg/kg, s.c, 4 semanas) y con BM-21 (4, 40 y 400 mg/kg, p.o) y se investigaron los efectos sobre tiempo de permanencia en la varilla rotatoria, potencial de acción muscular complejo (PAMC) del nervio ciático y sobre parámetros del estrés oxidativo.

Resultados: El BM-21 previno significativamente la aparición de los signos de neurotoxicidad conductual y las alteraciones de la amplitud y la velocidad del PAMC. La dosis inferior administrada (4 mg/kg) fue inefectiva, mientras que la máxima ensayada (400 mg/kg) mostró la mayor eficacia. A esta dosis fueron restauradas significativamente las concentraciones de hidroperóxidos totales y de glutatión reducido y la actividad de la superóxido dismutasa en el nervio ciático, cerebelo y cerebro y la de glutatión peroxidasa en nervio ciático

Conclusiones: El BM-21 administrado a dosis en que previno la neurotoxicidad inducida por ACR también indujo efectos antioxidantes en nervio ciático, cerebelo y cerebro, por lo que sus efectos neuroprotectores parecen estar mediados al menos parcialmente, por sus propiedades antioxidantes.

Palabras Clave: Axonopatía inducida por acrilamida; estrés oxidativo; BM-21; Thalassia testudinum.

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Citation Format: Roberto Menéndez, Teidy García, Anoland Garateix, Ruth A. Morales, Erik L. Regalado, Abilio Laguna, Olga Valdés, Miguel D. Fernández (2014) Neuroprotective and antioxidant effects of Thalassia testudinum extract BM-21, against acrylamide-induced neurotoxicity in mice. J Pharm Pharmacogn Res 2(3): 53-62.
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