A plant-based quadrocomplex for skin microbiota


J. Pharm. Pharmacogn. Res., vol. 10, no. 5, pp. 905-921, September-October 2022. DOI: https://doi.org/10.56499/jppres22.1452_10.5.905 Original Article In vitro and in vivo antimicrobial activity of an active plant-based quadrocomplex for skin hygiene [Actividad antimicrobiana in vitro e in vivo de un preparado vegetal activo para la higiene de la piel] Viktor A. Filatov1,2*, Olesya Yu. Kulyak1, Elena … Continue reading A plant-based quadrocomplex for skin microbiota

J. Pharm. Pharmacogn. Res., vol. 10, no. 5, pp. 905-921, September-October 2022.

DOI: https://doi.org/10.56499/jppres22.1452_10.5.905

Original Article

In vitro and in vivo antimicrobial activity of an active plant-based quadrocomplex for skin hygiene

[Actividad antimicrobiana in vitro e in vivo de un preparado vegetal activo para la higiene de la piel]

Viktor A. Filatov1,2*, Olesya Yu. Kulyak1, Elena I. Kalenikova1

1Department of Pharmaceutical Chemistry, Pharmacognosy and Organization of Pharmaceutical Business, Faculty of Basic Medicine, M. V. Lomonosov Moscow State University, Lomonosovsky Avenue, 27/1, Moscow, 119991, Russian Federation.

2Science Center, Department of Innovations and Product Development, Splat Global LLC, Novinsky boulevard, 8, Moscow, 121099, Russian Federation.

*E-mail: Viktor.Filatov@student.msu.ru; filatovviktor097@gmail.com


Context: The current epidemiological situation causes a new surge of interest to perspective antimicrobial formulations for proper skin hygiene.

Aims: To evaluate in vitro and in vivo the antimicrobial activity of a novel active quadrocomplex (QC) for skin hygiene based on Melaleuca alternifolia essential oil, eucalyptol, (-)-α-bisabolol and silver citrate. In addition, to analyze the phytochemical constituents by gas chromatography-mass spectrometry (GC-MS) and to assess the skin irritant potential after regular washing.

Methods: The phytochemical analysis was performed using GC-MS. The Minimum Inhibitory Concentrations (MICs) were assessed using a colony-counting method with resazurin. The type of pharmacological interaction was investigated using a modern checkerboard assay.

Results: The chemical composition exhibited 18 resolved phytochemicals with the highest concentrations for (-)-α-bisabolol (32.2%) and terpinen-4-ol (31.6%) through the GC-MS analysis. QC agents showed antimicrobial activity against Gram-positive and Gram-negative strains, with MIC values ranging from 1.25 to 40.00 mg/mL. The checkerboard assay demonstrated reduced MIC values for the combinations of QC agents against all reference strains. QC showed significant inhibition of Staphylococcus aureus growth with an average efficiency of 99.91% and Candida albicans 99.94 %. In vivo, the investigation of QC showed higher immediate and prolonged efficiency compared to base formulation (p<0.05). Dermatology evaluation indicated that QC added to the soap base did not affect the skin of hands during regular hand-washing and did not cause any negative effects on the skin.

Conclusions: QC exhibits a balanced performance between antimicrobial activity and biological safety and can be considered a promising bioactive composition for regular hand hygiene application. Therefore, additional investigations are needed to study the mechanism of antimicrobial activity.

Keywords: antimicrobial agents; drug synergism; Gram-negative pathogens; Gram-positive pathogens; phytochemicals; skin microbiota.


Contexto: La situación epidemiológica actual genera un nuevo interés por formular perspectivas antimicrobianas para una correcta higiene de la piel.

Objetivos: Evaluar in vitro e in vivo la actividad antimicrobiana de un novedoso quadrocomplejo (QC) activo para la higiene de la piel a base de aceite esencial de Melaleuca alternifolia, eucaliptol, (-)-α-bisabolol y citrato de plata. Además de analizar los constituyentes fitoquímicos por cromatografía de gases-espectrometría de masas (GC-MS) y evaluar el potencial irritante de la piel después del lavado regular.

Métodos: El análisis fitoquímico se realizó mediante GC-MS. Las concentraciones inhibitorias mínimas (CIM) se evaluaron utilizando un método de recuento de colonias con resazurina. El tipo de interacción farmacológica se investigó utilizando un ensayo de tablero de ajedrez moderno.

Resultados: La composición química mostró 18 fitoquímicos resueltos con las concentraciones más altas para (-)-α-bisabolol (32,2%) y terpinen-4-ol (31,6%) mediante el análisis GC-MS. Los agentes de control de calidad mostraron una actividad antimicrobiana contra las cepas Gram-positivas y Gram-negativas analizadas con valores de CIM que variaron de 1,25 a 40,00 mg/mL. El ensayo de tablero de ajedrez demostró valores de MIC reducidos para las combinaciones de agentes de control de calidad frente a todas las cepas de referencia. QC mostró una inhibición significativa del crecimiento de Staphylococcus aureus con una eficiencia promedio del 99,91% y Candida albicans 99,94 %. In vivo, la investigación de QC mostró una mayor eficacia inmediata y prolongada en comparación con la formulación base (p<0,05). La evaluación dermatológica indicó que QC agregado a la base de jabón no afectó la piel de las manos durante el lavado regular de manos y no causó ningún efecto negativo en la piel.

Conclusiones: QC exhibe un rendimiento equilibrado entre la actividad antimicrobiana y la seguridad biológica y puede considerarse una composición bioactiva prometedora para la aplicación regular de higiene de manos. Por lo tanto, se necesitan investigaciones adicionales para estudiar el mecanismo de la actividad antimicrobiana.

Palabras Clave: agentes antimicrobianos; fitoquímicos; microbiota de la piel; patógenos Gram-negativo; patógenos Gram-positivo; sinergismo de fármacos.

Citation Format: Filatov VA, Kulyak OY, Kalenikova EI (2022) In vitro and in vivo antimicrobial activity of an active plant-based quadrocomplex for skin hygiene. J Pharm Pharmacogn Res 10(5): 905–921. https://doi.org/10.56499/jppres22.1452_10.5.905

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