Achillea biebersteinii essential oil activity

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J Pharm Pharmacogn Res 8(2): 155-166, 2020. DOI: https://doi.org/10.56499/jppres19.746_8.2.155 Original Article Antibacterial and antibiofilm activity of essential oil of Achillea biebersteinii and its mode of action [Actividad antibacteriana y anti-biopelícula del aceite esencial de Achillea biebersteinii y su modo de acción] Jehad M. Al-Shuneigat1*, Sameeh A. Al- Sarayreh1, Mahmoud A. Al-Qudah2, Yousef M. Al–Saraireh3 1Faculty of Medicine, … Continue reading Achillea biebersteinii essential oil activity

J Pharm Pharmacogn Res 8(2): 155-166, 2020.

DOI: https://doi.org/10.56499/jppres19.746_8.2.155

Original Article

Antibacterial and antibiofilm activity of essential oil of Achillea biebersteinii and its mode of action

[Actividad antibacteriana y anti-biopelícula del aceite esencial de Achillea biebersteinii y su modo de acción]

Jehad M. Al-Shuneigat1*, Sameeh A. Al- Sarayreh1, Mahmoud A. Al-Qudah2, Yousef M. Al–Saraireh3

1Faculty of Medicine, Department of Biochemistry and Molecular Biology Mutah University, Mutah, Jordan.
2Faculty of Science, Department of Chemistry Yarmouk University, Irbid, Jordan.
3Faculty of Medicine, Department of Pharmacology Mutah University, Mutah, Jordan.
Abstract

Context: Microbial biofilms and antibiotic-resistant bacterial strains pose a challenge in clinical world as they fail to respond to conventional treatments. This failure of antibiotic treatment led researchers to look for alternatives. A possible alternative is plants derived essential oils. Many studies have reported that certain essential oils succeed where antibiotics fail.

Aims: To test antibacterial and antibiofilm activities of essential oil of Achillea biebersteinii and its mode of action.

Methods: Minimum biofilm inhibitory concentration (MBIC) susceptibility assays were performed using a biofilm inoculator with a 96-well plate with peg led. Minimum inhibitory concentration (MIC) was determined in normal microtitre plates using a twofold dilution series.

Results: Achillea biebersteinii essential oil showed good activity against all tested bacteria. The MIC values were in the range of 0.125 - 1 mg/mL while MBIC values were in the range of 0.125 – 4 mg/mL. The mechanism of action of Achillea biebersteinii essential oil is related to a strong increase in membrane permeability of 260 nm absorbing materials and potassium ions from the cell membrane. Achillea biebersteinii essential oil was able to inhibit initial adherence of methicillin-resistant Staphylococcus aureus (ATCC 43300) at sub-inhibitory concentrations.

Conclusions: Achillea biebersteinii essential oil has the potential for use as an effective antibacterial and antibiofilm agent that functions by impairing cell membrane permeability resulting in cellular death.

Keywords: Achillea biebersteinii; antibacterial; antibiofilm; mode of action.

Resumen

Contexto: Las biopelículas microbianas y las cepas bacterianas resistentes a los antibióticos representan un desafío en el mundo clínico, ya que no responden a los tratamientos convencionales. Este fracaso del tratamiento con antibióticos llevó a los investigadores a buscar alternativas. Una posible alternativa son los aceites esenciales derivados de plantas. Muchos estudios han informado que ciertos aceites esenciales tienen éxito donde los antibióticos fallan.

Objetivos: Evaluar las actividades antibacteriana y antibiopelícula del aceite esencial de Achillea biebersteinii y su modo de acción.

Métodos: Los ensayos de susceptibilidad de concentración mínima inhibitoria de biopelícula (MBIC) se realizaron utilizando un inoculador de biopelícula con una placa de 96 pocillos con clavija led. La concentración inhibitoria mínima (MIC) se determinó en placas de microtitulación normales usando una serie de dilución doble.

Resultados: El aceite esencial de Achillea biebersteinii mostró buena actividad contra todas las bacterias probadas. Los valores de MIC estuvieron en el rango de 0.125 - 1 mg/mL mientras que los valores de MBIC estuvieron en el rango de 0.125 - 4 mg/mL. El mecanismo de acción del aceite esencial Achillea biebersteinii está relacionado con un fuerte aumento en la permeabilidad de la membrana de los materiales absorbentes de 260 nm y los iones de potasio de la membrana celular. El aceite esencial de Achillea biebersteinii fue capaz de inhibir la adherencia inicial de Staphylococcus aureus resistente a meticilina (ATCC 43300) en concentraciones subinhibitorias.

Conclusiones El aceite esencial de Achillea biebersteinii tiene el potencial de usarse como un agente antibacteriano y antibiopelícula eficaz que funciona al afectar la permeabilidad de la membrana celular y provocar la muerte celular.

Palabras Clave: Achillea biebersteinii; antibacteriano; antibiopelícula; modo de acción.

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Citation Format: Al-Shuneigat JM, Al- Sarayreh SA, Al-Qudah MA, Al–Saraireh YM (2020) Antibacterial and antibiofilm activity of essential oil of Achillea biebersteinii and its mode of action. J Pharm Pharmacogn Res 8(2): 155–166. DOI: https://doi.org/10.56499/jppres19.746_8.2.155

© 2020 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

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